Immunoprecipitation of ECHS1 protein from 293T whole cell extracts using 5 μg of ECHS1 antibody (GTX114375).
Western blot analysis was performed using ECHS1 antibody (GTX114375).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

ECHS1 antibody detects ECHS1 protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ECHS1 antibody (GTX114375) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ECHS1 antibody detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ECHS1 antibody (GTX114375) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ECHS1 antibody detects ECHS1 protein by Western blot analysis.
A. 30 μg NT2D1 whole cell lysate/extract
B. 30 ug PC-3 whole cell lysate/extract
C. 30 ug U87-MG whole cell lysate/extract
D. 30 ug SK-N-SH whole cell lysate/extract
12 % SDS-PAGE
ECHS1 antibody (GTX114375) dilution: 1:3000

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ECHS1 antibody (GTX114375) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ECHS1 antibody detects ECHS1 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
12 % SDS-PAGE
ECHS1 antibody (GTX114375) dilution: 1:1000

ECHS1 antibody detects ECHS1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ECHS1 protein stained by ECHS1 antibody (GTX114375) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.