Wild-type (WT) and EEF1E1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EEF1E1 antibody [N1C3] (GTX105658) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX105658 diluted at 1:1000

EEF1E1 antibody [N1C3] detects EEF1E1 protein at cytoplasm on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
EEF1E1 antibody [N1C3] (GTX105658) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

EEF1E1 antibody [N1C3] detects EEF1E1 protein at cytoplasm on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
EEF1E1 antibody [N1C3] (GTX105658) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
12% SDS PAGE
GTX105658 diluted at 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using EEF1E1(GTX105658) antibody at 1:200 dilution.