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EGF antibody [EGF-10]

Cat. No. GTX10409

Host

Mouse

Clonality

Monoclonal

Clone Name

EGF-10

Isotype

IgG1

Application

WB, IHC-P, FACS, Dot, ELISA, Neutralizing/Inhibition, RIA

Reactivity

Human
Package
100 μl ($499)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
FACS Assay dependent
Dot 0.1 μg/ml
ELISA Assay dependent
Neutralizing/Inhibition Assay dependent
RIA Assay dependent
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Positive Control

recombinant human EGF

Product Note

Specifically reacts with both native and recombinant human EGF. The antibody does not cross-react with mouse EGF.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

recombinant human EGF

Purification

Purified immunoglobulin

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epidermal growth factor , HOMG4 , URG

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Epidermal growth factor (EGF) is a single chain poly-peptide hormone (6 kDa) which was first isolated from the submandibular gland of the mouse and is a potent growth-promoting factor for a variety of tissue cells in vivo and in vitro. Human EGF (hEGF) has also been extracted from human urine and shown to be identical or closely related to -urogastrone, a potent inhibitor of gastric acid secretion. Human EGF is structurally homologous to human transforming growth factor-alpha (TGF-Alpha) which also exerts its actions through EGF receptors. It is homologous to a sequence contained in a 19 kDa protein of Vaccinia virus, which appears to utilize the EGF receptor to gain entry into cells. Cloning data for mouse and human EGF suggest that the 53 amino-acid EGF peptide is cleaved from a 125-130 kDa, 1217 amino-acid precursor containing an N-terminal 29 amino-acid signal sequence. Mouse and human EGF exhibit 70% similarity at the amino acid level and are highly species cross-reactive. Structures obtained for both mouse and human EGF suggest that the protein exists largely in a beta sheet conformation, with a C-terminal hairpin that may be involved in receptor binding. EGF acts through a specific cell surface receptor glycoprotein of approximately 170 kDa. EGF binding induces phosphatidylinositide hydrolysis and generation of diacylglycerol (DAG). DAG-dependent activation of protein kinase C, in turn, results in the phosphorylation of a threonine residue at position 654 of the receptor, in attenuation of the intrinsic tyrosine kinase activity, and subsequently alterations of receptor homeostasis. Cellular metabolic effects of EGF include stimulation of ion fluxes, glucose transport, glycolysis and synthesis of DNA, RNA and proteins. EGF acts on a variety of tissues and binding has been demonstrated in virtually all cell types tested, with the exception of those of the hematopoietic lineage. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa and SV40-3T3 cells. Human EGF is found in body fluids such as urine, milk, saliva, sweat, and seminal fluid and has its highest concentration in alpha granules of blood platelets. It has a widespread distribution in human tissues and organs, including neoplasms. The biological role of EGF includes the inhibition of gastric acid secretion, support of growth and differentiation during fetal development, neuromodulation in the central nervous system and stimulation of epidermal growth and keratinization. Since EGF plays a role in the proliferation and/ or differentiation of normal as well as tumor cells derived from a variety of tissues, 5 and can contribute to pathological states, an in vitro assay for its detection and quantification is desirable. Monoclonal antibody reacting specifically with EGF may be used in the determination and quantification of the molecule in many in vitro systems and in vivo human models, and for studies on structure-function relationship of this factor in binding to the receptor molecule.

Database

Research Area

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 499