Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with EGFRvIII antibody [HL2541] (GTX638910) diluted at 1:5000 and competitor's antibody (Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with EGFRvIII antibody [HL2541] (GTX638910) diluted at 1:5000 and competitor's antibody (Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (GTX638910) diluted at 1:500 and competitor's antibody (highly cited competitor) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (GTX638910) diluted at 1:500 and competitor's antibody (highly cited competitor) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFRvIII antibody [HL2541] (GTX638910) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunohistochemical analysis.
Sample: EGFRvIII transfected 293T cell FFPE Cell Pellet Block.
Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (GTX638910) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (GTX638910) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).