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EIF4E2 antibody [N1C3]

KO/KD
Anti-EIF4E2 antibody [N1C3] used in Western Blot (WB). GTX103977
Anti-EIF4E2 antibody [N1C3] used in Western Blot (WB). GTX103977
Anti-EIF4E2 antibody [N1C3] used in Western Blot (WB). GTX103977
Anti-EIF4E2 antibody [N1C3] used in Western Blot and Immunoprecipitation (WB IP). GTX103977

Cat. No. GTX103977

Reference 18

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all EIF4E2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Predict Reactivity

Zebrafish, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF4E2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036842

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 4E family member 2 , 4E-LP , 4EHP , EIF4EL3 , IF4e , h4EHP

Background

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

Database

Research Area

DATA IMAGES

Anti-EIF4E2 antibody [N1C3] used in Western Blot (WB). GTX103977

GTX103977 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with EIF4E2 antibody [N1C3] (GTX103977) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EIF4E2 antibody [N1C3] used in Western Blot (WB). GTX103977

GTX103977 WB Image

KO/KD

The data was published in the journal Nat Commun in 2019. PMID: 30902983

Anti-EIF4E2 antibody [N1C3] used in Western Blot (WB). GTX103977

GTX103977 WB Image

KO/KD

The data was published in the journal Elife in 2018. PMID: 29412140

Anti-EIF4E2 antibody [N1C3] used in Western Blot and Immunoprecipitation (WB IP). GTX103977

GTX103977 WB IP Image

The data was published in the journal Nat Commun in 2019. PMID: 30902983

REFERENCE

Application Reference

Naeli P et al. J Cell Sci 2023; 136 (19) The SARS-CoV-2 protein NSP2 enhances microRNA-mediated translational repression.
Wiebe S et al. Mol Brain 2023; 16 (1) : 9 Cell-type-specific translational control of spatial working memory by the cap-binding protein 4EHP.
Application : WB
Reactivity : Mouse
Phaedra R Theodoridis et al. Proc Natl Acad Sci U S A 2021; 118 (7) Local translation in nuclear condensate amyloid bodies.
Application : WB
Reactivity : Human
M?ndez-Sol?s O et al. Cell Rep 2021; 37 (13) : 110144 Kaposi's sarcoma herpesvirus activates the hypoxia response to usurp HIF2α-dependent translation initiation for replication and oncogenesis.
Application : WB
Reactivity : Human
Wiebe S et al. Mol Autism 2020; 11 (1) : 92 The eIF4E homolog 4EHP (eIF4E2) regulates hippocampal long-term depression and impacts social behavior.
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REVIEW

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Application Reference
Naeli P et al. J Cell Sci 2023; 136 (19) The SARS-CoV-2 protein NSP2 enhances microRNA-mediated translational repression.
Wiebe S et al. Mol Brain 2023; 16 (1):9 Cell-type-specific translational control of spatial working memory by the cap-binding protein 4EHP.
Application : WB
Reactivity : Mouse
Phaedra R Theodoridis et al. Proc Natl Acad Sci U S A 2021; 118 (7) Local translation in nuclear condensate amyloid bodies.
Application : WB
Reactivity : Human
M?ndez-Sol?s O et al. Cell Rep 2021; 37 (13):110144 Kaposi's sarcoma herpesvirus activates the hypoxia response to usurp HIF2α-dependent translation initiation for replication and oncogenesis.
Application : WB
Reactivity : Human
Wiebe S et al. Mol Autism 2020; 11 (1):92 The eIF4E homolog 4EHP (eIF4E2) regulates hippocampal long-term depression and impacts social behavior.
Juszkiewicz S et al. Elife 2020; 9 Ribosome collisions trigger cis-acting feedback inhibition of translation initiation.
Pringle ES et al. Viruses 2019; 12 (1) The Zebrafish Xenograft Platform-A Novel Tool for Modeling KSHV-Associated Diseases.
Eric S. Pringle et al. virology 2019; (Epub) The Zebrafish Xenograft Platform – A Novel Tool for Modeling KSHV-Associated Diseases.
Jeong SJ et al. Nat Commun 2019; 10 (1):1357 A threonyl-tRNA synthetase-mediated translation initiation machinery.
Application : WB
Reactivity : Human
Kelly NJ et al. Oncogene 2018; 37 (5):651-662 Hypoxia activates cadherin-22 synthesis via eIF4E2 to drive cancer cell migration, invasion and adhesion.
Jafarnejad SM et al. Elife 2018; 7 Translational control of ERK signaling through miRNA/4EHP-directed silencing.
Application : WB
Reactivity : Human
Garzia A et al. Nat Commun 2017; 8:16056 The E3 ubiquitin ligase and RNA-binding protein ZNF598 orchestrates ribosome quality control of premature polyadenylated mRNAs.
Application : WB
Reactivity : Human
Chapat C et al. Proc Natl Acad Sci U S A 2017; 114 (21):5425-5430 Cap-binding protein 4EHP effects translation silencing by microRNAs.
Sese M et al. Oncotarget 2017; 8 (70):114856-114876 Hypoxia-mediated translational activation of ITGB3 in breast cancer cells enhances TGF-β signaling and malignant features in vitro and in vivo.
Application : WB
Reactivity : Human
Fu R et al. RNA 2016; 22 (3):373-82 Recruitment of the 4EHP-GYF2 cap-binding complex to tetraproline motifs of tristetraprolin promotes repression and degradation of mRNAs with AU-rich elements.
Application : WB
Reactivity : Mouse
Timpano S et al. J Biol Chem 2016; (Epub) Human Cells Cultured Under Physiological Oxygen Utilize Two Cap-binding Proteins to Recruit Distinct mRNAs for Translation.
Application : WB
Reactivity : Human
Uniacke J et al. Cancer Res 2014; Cancer Cells Exploit eIF4E2-Directed Synthesis of Hypoxia Response Proteins to Drive Tumor Progression.
Application : WB
Reactivity : Human
Morita M et al. Mol Cell Biol 2012; 32 (17):3585-93 A novel 4EHP-GIGYF2 translational repressor complex is essential for mammalian development.
Application : WB
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169