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EIF4E2 antibody [N1C3]

Anti-EIF4E2 antibody [N1C3] used in Western Blot (WB). GTX103977
Anti-EIF4E2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103977

Cat No. GTX103977

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

Target recombinant protein

Predict Reactivity

Zebrafish, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF4E2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

eukaryotic translation initiation factor 4E family member 2 , 4E-LP , 4EHP , EIF4EL3 , IF4e , h4EHP

Background

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

Database

Research Area

DATA IMAGES

Anti-EIF4E2 antibody [N1C3] used in Western Blot (WB). GTX103977

GTX103977 WB Image

The WB analysis of EIF4E2 antibody was published by Jafarnejad SM and colleagues in the journal Elife in 2018.PMID: 29412140

Anti-EIF4E2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103977

GTX103977 IHC-P Image

EIF4E2 antibody [N1C3] detects EIF4E2 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
EIF4E2 antibody [N1C3] (GTX103977) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169