EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytosol on mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
EIF4G3 antibody [N1], N-term (GTX118109) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

EIF4G3 antibody [N1], N-term detects EIF4G3 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: EIF4G3 protein stained by EIF4G3 antibody [N1], N-term (GTX118109) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
EIF4G3 stained by EIF4G3 antibody [N1], N-term (GTX118109) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EIF4G3 antibody [N1], N-term (GTX118109) diluted at 1:10000.

Immunoprecipitation of EIF4G3 protein from 293T whole cell extracts using 5 μg of EIF4G3 antibody [N1], N-term (GTX118109).
Western blot analysis was performed using EIF4G3 antibody [N1], N-term (GTX118109).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytosol on mouse brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain stem.
EIF4G3 antibody [N1], N-term (GTX118109) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EIF4G3 protein stained by EIF4G3 antibody [N1], N-term (GTX118109) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with EIF4G3 antibody [N1], N-term (GTX118109) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
EIF4G3 stained by EIF4G3 antibody [N1], N-term (GTX118109) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
EIF4G3 stained by EIF4G3 antibody [N1], N-term (GTX118109) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
EIF4G3 antibody [N1], N-term (GTX118109) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min