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ENOSF1 antibody [N1C2]

Anti-ENOSF1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119464
Anti-ENOSF1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX119464
Anti-ENOSF1 antibody [N1C2] used in Western Blot (WB). GTX119464

Cat No. GTX119464

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ENOSF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617332

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

enolase superfamily member 1 , FUCD , RTS , TYMSAS

Cellular Localization

Mitochondrion

Background

This gene was originally identified as a naturally occurring antisense transcript to the human thymidylate synthase gene. Alternate splice variants have been described, one of which (named rTSalpha) represents an alternate 3'UTR that is complementary to the 3'UTR and terminal intron of the thymidylate synthase (TS) RNA and down-regulates TS expression. Another transcript variant (named rTSalpha) does not overlap the TS locus. The function of this gene appears to be primarily to regulate expression of the TS locus both via the antisense transcript as well as through the encoded proteins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ENOSF1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119464

GTX119464 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using ENOSF1(GTX119464) antibody at 1:500 dilution.

Anti-ENOSF1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX119464

GTX119464 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using ENOSF1(GTX119464) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ENOSF1 antibody [N1C2] used in Western Blot (WB). GTX119464

GTX119464 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX119464 diluted at 1:1000

REFERENCE

REVIEW

ENOSF1 antibody [N1C2] Cat No. GTX119464

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 29127306
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 29127306
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169