GeneTex
United States (US)

ENPP1 antibody [N2C2], Internal

GTX103447_41675_20150605_WB_19011506_430.jpg
GTX103447_42123_20150618_WB_liver_19011506_575.jpg

Cat No. GTX103447

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

Positive Control

HepG2 , A549 , human liver

Predict Reactivity

Rabbit, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.14mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ENPP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ARHR2 antibody, M6S1 antibody, NPP1 antibody, NPPS antibody, PC-1 antibody, PCA1 antibody, PDNP1 antibody, ENPP1 antibody, alkaline phosphodiesterase 1 antibody, phosphodiesterase I/nucleotide pyrophosphatase 1 antibody, E-NPP 1 antibody, plasma-cell membrane glycoprotein 1 antibody, plasma-cell membrane glycoprotein PC-1 antibody, "membrane component, chromosome 6, surface marker 1 antibody", membrane component chromosome 6 surface marker 1 antibody, Ly-41 antigen antibody, ectonucleotide pyrophosphatase/phosphodiesterase family member 1 antibody, ectonucleotide pyrophosphatase/phosphodiesterase 1 antibody

Background

This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103447_41675_20150605_WB_19011506_430.jpg

GTX103447 WB Image

ENPP1 antibody detects ENPP1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ENPP1 antibody (GTX103447) at a dilution of 1:500.

GTX103447_42123_20150618_WB_liver_19011506_575.jpg

GTX103447 WB Image

ENPP1 antibody detects ENPP1 protein by Western blot analysis. Human tissue extracts (30 μg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with ENPP1 antibody (GTX103447) at a dilution of 1:1000.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169