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ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173]

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] used in Western Blot (WB). GTX635617
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] used in Western Blot (WB). GTX635617
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] used in Western Blot (WB). GTX635617
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] used in Western Blot (WB). GTX635617

Cat No. GTX635617

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL173

Isotype

IgG

Application

WB

Reactivity

Human, Mouse, Rat
Package
100 μl ($369),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

Neuro2A (50 μM Ethacrynic acid treatment for 2 hr) , A431 (starvation for 16 hr) , A431 (starvation for 16 hr and 200 ng/ml EGF treatment for 0.5 hr)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888542

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mitogen-activated protein kinase 3 , ERK-1 , ERK1 , ERT2 , HS44KDAP , HUMKER1A , P44ERK1 , P44MAPK , PRKM3 , p44-ERK1 , p44-MAPK

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] used in Western Blot (WB). GTX635617

GTX635617 WB Image

Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] used in Western Blot (WB). GTX635617

GTX635617 WB Image

Untreated (–) and treated (+) Rat2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] used in Western Blot (WB). GTX635617

GTX635617 WB Image

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] used in Western Blot (WB). GTX635617

GTX635617 WB Image

Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 369
$ 169
Custom Conjugations