APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody detects Erk1 and Erk2 when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), and singly phosphorylated at Tyr204.
Predict Reactivity
Zebrafish, Bovine, Dog, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Thr202/Tyr204 of human ERK1. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888542
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mitogen-activated protein kinase 3 , ERK-1 , ERK1 , ERT2 , HS44KDAP , HUMKER1A , P44ERK1 , P44MAPK , PRKM3 , p44-ERK1 , p44-MAPK
Cellular Localization
Cytoplasm,Nucleus
Background
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX635617 WB Image
Wild-type (WT) and ERK knockout (KO) 293T cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635617 WB Image
Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX635617 WB Image
Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX635617 WB Image
Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635617 WB Image
Untreated (–) and treated (+) Rat2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635617 WB Image
Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635617 ICC/IF Image
ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] detects ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) protein at endoplasmic reticulum and cytoplasm by immunofluorescent analysis. Sample: NIH-3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) stained by ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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GTX635617 WB Image
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:500 and competitor's antibody (# Highly Cited Antibody) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX635617 WB Image
Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:500 and competitor's antibody (# Highly Cited Antibody) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX635617 WB Image
Drosophila tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX635617 WB Image
Untreated (–) and treated (+) A431 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635617 ICC/IF Image
ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] detects ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) protein by immunofluorescent analysis.Sample: Mock and treated A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) stained by ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX635617 IHC-P Image
ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] detects ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) stained by ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
REVIEW
ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173]
Cat. No. GTX635617
Rating
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( Average 5 based on 1 users reviews)
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Application
Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 18-Apr-2023
Application Tested :
WB
Sample Species :
Ms
Sample :
NIH3T3 treated with or without 5ng/ml TGFb (24h)
Amount used :
30ug
Blocking :
0.05% BSA in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr