APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain , *SD Rat brain thalamus , *SD Rat brain amygdala
Specificity/Sensitivity
IP/MS validation was supported by references (PMID:30377410)
Predict Reactivity
Zebrafish, Bovine, Dog, Chicken, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.49 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ERK2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Mitogen-Activated Protein Kinase 1,Erk,Erk-2,Erk2,Ert1,Mapk2,P42Mapk,Prkm1,Prkm2,P38,P40,P41,P41Mapk,P42-Mapk,Mapk1
Background
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX113094 WB Image
Wild-type (WT) and ERK2 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK2 antibody [N2C3] (GTX113094) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by 
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GTX113094 IP Image
ERK2 antibody [N2C3] immunoprecipitates MAPK1 protein in IP experiments.
IP samples: HeLa whole cell extract
A. 30 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MAPK1 protein by 4 μg ERK2 antibody [N2C3] (GTX113094)
10 % SDS-PAGE
The immunoprecipitated MAPK1 protein was detected by ERK2 antibody [N2C3] (GTX113094) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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GTX113094 WB Image
Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX113094 diluted at 1:5000
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GTX113094 WB Image
Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
10% SDS PAGE
GTX113094 diluted at 1:1000
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GTX113094 IHC-P Image
ERK2 antibody [N2C3] detects ERK2 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ERK2 antibody [N2C3] (GTX113094) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113094 IHC-P Image
ERK2 antibody [N2C3] detects ERK2 protein at cytosol and nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
ERK2 antibody [N2C3] (GTX113094) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
REVIEW
ERK2 antibody [N2C3]
Cat No. GTX113094
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Rating
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( Average 5 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 22-Oct-2012
Application Tested :
WB
Sample Species :
Rat
Sample :
SD Rat brain thalamus tissue,amygdala tissue
Amount used :
20μg
Blocking :
5% milk in TBST, 25°C, 1Hr
Primary Antibody :
1:5000, 4°C, overnightHr
