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ERK2 antibody [N2C3]

KO/KD IP/MS
Anti-ERK2 antibody [N2C3] used in Western Blot (WB). GTX113094
Anti-ERK2 antibody [N2C3] used in Immunoprecipitation (IP). GTX113094
Anti-ERK2 antibody [N2C3] used in Western Blot (WB). GTX113094
Anti-ERK2 antibody [N2C3] used in Western Blot (WB). GTX113094
Anti-ERK2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113094
Anti-ERK2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113094

Cat No. GTX113094

Reference 10
1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human, Mouse, Rat, Pig
Package
100μl ($319),
25μl ($169)
See all ERK2 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain , *SD Rat brain thalamus , *SD Rat brain amygdala

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377410)

Predict Reactivity

Rat, Zebrafish, Bovine, Dog, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mitogen-Activated Protein Kinase 1,Erk,Erk-2,Erk2,Ert1,Mapk2,P42Mapk,Prkm1,Prkm2,P38,P40,P41,P41Mapk,P42-Mapk,Mapk1

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-ERK2 antibody [N2C3] used in Western Blot (WB). GTX113094

GTX113094 WB Image

KO/KD

Wild-type (WT) and ERK2 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK2 antibody [N2C3] (GTX113094) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The CRISPR knockout cell line was generated by EdiGene Inc.

Anti-ERK2 antibody [N2C3] used in Immunoprecipitation (IP). GTX113094

GTX113094 IP Image

ERK2 antibody [N2C3] immunoprecipitates MAPK1 protein in IP experiments.
IP samples: HeLa whole cell extract
A. 30 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MAPK1 protein by 4 μg ERK2 antibody [N2C3] (GTX113094)
10 % SDS-PAGE
The immunoprecipitated MAPK1 protein was detected by ERK2 antibody [N2C3] (GTX113094) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-ERK2 antibody [N2C3] used in Western Blot (WB). GTX113094

GTX113094 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX113094 diluted at 1:5000

Anti-ERK2 antibody [N2C3] used in Western Blot (WB). GTX113094

GTX113094 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
10% SDS PAGE
GTX113094 diluted at 1:1000

Anti-ERK2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113094

GTX113094 IHC-P Image

ERK2 antibody [N2C3] detects ERK2 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ERK2 antibody [N2C3] (GTX113094) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Anti-ERK2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113094

GTX113094 IHC-P Image

ERK2 antibody [N2C3] detects ERK2 protein at cytosol and nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
ERK2 antibody [N2C3] (GTX113094) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
REFERENCE

Application Reference

Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Chen CF et al. Oncology Letters 2018; : 6323-6330 synergistic inhibitory effects of * and curcumin on human *-resistant oral cancer car cells through intrinsic apoptotic process.
Application : WB
Reactivity : Human
Nana AW et al. Horm Cancer 2018; (Epub ahead of print) Nano-Diamino-Tetrac (NDAT) Enhances Resveratrol-Induced Antiproliferation by Action on the RRM2 Pathway in Colorectal Cancers.
Application : WB
Reactivity : Human
Jui-Cheng Tsai et al. Journal of Functional Foods 2018; 48 : 283-296 Extracts of Cerbera manghas L. effectively inhibit the viability of glioblastoma cell lines and their cancer stemloids in vitro and in mouse xenograft model.
Application : WB
Reactivity : Human
Cai H et al. J Nat Med 2017; (Epub ahead of print) 18β-glycyrrhetinic acid inhibits migration and invasion of human gastric cancer cells via the ROS/PKC-α/ERK pathway.
Application : WB
Reactivity : Human
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REVIEW

ERK2 antibody [N2C3] Cat No. GTX113094

Rating
( Average 5 based on 1 users reviews)
Application
Anti-ERK2 antibody [N2C3] used in Western Blot (WB). GTX113094
Date : Anonymous submitted on 22-Oct-2012
Score :
Application Tested : WB
Sample Species : Rat
Sample : SD Rat brain thalamus tissue,amygdala tissue
Amount used : 20μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, overnightHr
Submit a Review
Application Reference
Sikorski K et al. Nat Methods 2018; 15 (11):909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Chen CF et al. Oncology Letters 2018;:6323-6330 synergistic inhibitory effects of * and curcumin on human *-resistant oral cancer car cells through intrinsic apoptotic process.
Application : WB
Reactivity : Human
Nana AW et al. Horm Cancer 2018; (Epub ahead of print) Nano-Diamino-Tetrac (NDAT) Enhances Resveratrol-Induced Antiproliferation by Action on the RRM2 Pathway in Colorectal Cancers.
Application : WB
Reactivity : Human
Jui-Cheng Tsai et al. Journal of Functional Foods 2018; 48:283-296 Extracts of Cerbera manghas L. effectively inhibit the viability of glioblastoma cell lines and their cancer stemloids in vitro and in mouse xenograft model.
Application : WB
Reactivity : Human
Cai H et al. J Nat Med 2017; (Epub ahead of print) 18β-glycyrrhetinic acid inhibits migration and invasion of human gastric cancer cells via the ROS/PKC-α/ERK pathway.
Application : WB
Reactivity : Human
Chin YT et al. Mediators Inflamm 2016; 2016:6953459 2,3,5,4'-Tetrahydroxystilbene-2-O-β-glucoside Isolated from Polygoni Multiflori Ameliorates the Development of Periodontitis.
Application : WB
Reactivity : Human
Lin HY et al. Steroids 2016; (Epub ahead of print) Actions of l-thyroxine and Nano-diamino-tetrac (Nanotetrac) on PD-L1 in cancer cells.
Application : WB
Reactivity : Human
Sinanoglu O et al. Urology 2012; 80 (6):1392.e1-6 The protective effects of spirulina in * induced nephrotoxicity and urotoxicity in rats.
Application : WB
Reactivity : Mouse
Tseng HL et al. Toxicol Appl Pharmacol 2012; 264 (1):104-13 Quercetin 3-O-methyl ether protects FL83B cells from copper induced oxidative stress through the PI3K/Akt and MAPK/Erk pathway.
Application : WB
Reactivity : Mouse
Yun-Hong Y et al. Mol Cell Proteomics 2011; 10 (10):M110.007138 A study of the spatial protein organization of the postsynaptic density isolated from porcine cerebral cortex and cerebellum.
Application : WB
Reactivity : Pig
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169