Wild-type (WT) and ERK2 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK2 antibody [N2C3] (GTX113094) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX113094 diluted at 1:5000

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
10% SDS PAGE
GTX113094 diluted at 1:1000

ERK2 antibody [N2C3] detects ERK2 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ERK2 antibody [N2C3] (GTX113094) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ERK2 antibody [N2C3] immunoprecipitates MAPK1 protein in IP experiments.
IP samples: HeLa whole cell extract
A. 30 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MAPK1 protein by 4 μg ERK2 antibody [N2C3] (GTX113094)
10 % SDS-PAGE
The immunoprecipitated MAPK1 protein was detected by ERK2 antibody [N2C3] (GTX113094) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

ERK2 antibody [N2C3] detects ERK2 protein at cytosol and nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
ERK2 antibody [N2C3] (GTX113094) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ERK2 antibody detects ERK2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat mammary gland.
ERK2 stained by ERK2 antibody (GTX113094) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERK2 antibody (GTX113094) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERK2 antibody (GTX113094) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Cancers (Basel) in 2019. PMID: 31835799

The data was published in the journal Cancers (Basel) in 2019. PMID: 31835799