Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ERP29(GTX102225) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

ERP29 antibody detects ERP29 protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
ERP29 antibody (GTX102225) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ERP29 antibody detects ERP29 protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
ERP29 antibody (GTX102225) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using ERP29(GTX102225) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ERP29 antibody detects ERP29 protein at cytosol on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
ERP29 antibody (GTX102225) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 μg of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
12% SDS PAGE
GTX102225 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 μg of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX102225 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.