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ERR alpha antibody [N1], N-term

Anti-ERR alpha antibody [N1], N-term used in Immunoprecipitation (IP). GTX108166
Anti-ERR alpha antibody [N1], N-term used in Western Blot (WB). GTX108166
Anti-ERR alpha antibody [N1], N-term used in ChIP assay (ChIP assay). GTX108166
Anti-ERR alpha antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108166
Anti-ERR alpha antibody [N1], N-term used in Western Blot (WB). GTX108166
Anti-ERR alpha antibody [N1], N-term used in Western Blot (WB). GTX108166
Anti-ERR alpha antibody [N1], N-term used in Western Blot (WB). GTX108166

Cat No. GTX108166

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP, ChIP assay

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

293T , A431 , H1299 , Mouse kidney , Rat kidney , *A549

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human ERR alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

estrogen related receptor alpha , ERR1 , ERRa , ERRalpha , ESRL1 , NR3B1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. A processed pseudogene of ESRRA is located on chromosome 13q12.1. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ERR alpha antibody [N1], N-term used in Immunoprecipitation (IP). GTX108166

GTX108166 IP Image

ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of ERR alpha protein by 2 μg of ERR alpha antibody (GTX108166) 7.5% SDS-PAGE The immunoprecipitated ERR alpha protein was detected by ERR alpha antibody (GTX108166) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ERR alpha antibody [N1], N-term used in Western Blot (WB). GTX108166

GTX108166 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
C:H1299
12% SDS PAGE
GTX108166 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERR alpha antibody [N1], N-term used in ChIP assay (ChIP assay). GTX108166

GTX108166 ChIP assay Image

Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-ERR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter or GAPDH promoter.

Anti-ERR alpha antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108166

GTX108166 IHC-P Image

ERR alpha antibody [N1], N-term detects ERR alpha protein at nucleus on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ERR alpha antibody [N1], N-term used in Western Blot (WB). GTX108166

GTX108166 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ERR alpha antibody [N1], N-term used in Western Blot (WB). GTX108166

GTX108166 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERR alpha antibody [N1], N-term used in Western Blot (WB). GTX108166

GTX108166 WB Image

The WB analysis of ERR alpha antibody [N1] was published by Carnesecchi J and colleagues in the journal PLoS One in 2015.PMID: 25781607

REFERENCE

REVIEW

ERR alpha antibody [N1], N-term Cat No. GTX108166

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 28-Mar-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 40μg
Blocking : 5% milk, 23°C, 1Hr
Primary Antibody : 1:3000, 4°C, 16Hr
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169