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EasyBlot anti Mouse IgG (HRP)

EasyBlot anti Mouse IgG (HRP). GTX221667-01
EasyBlot anti Mouse IgG (HRP). GTX221667-01

Cat. No. GTX221667-01

Reference 36
1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Mouse
Package
250 μl ($199),
50 μl ($89)
See all Mouse IgG products
PRODUCT

Summary

EasyBlot anti-Mouse IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of mouse IgG and does not bind the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP). EasyBlot is simple to use and results in Western Blot detection of only the target band in your IP experiments. Exercise caution when using Protein A/G for IP with EasyBlot. Protein A/G contaminants bind to mouse IgG with high affinity and may cause contaminating bands with a molecular weight similar to IgG heavy chain or light chian.

To minimize background from Protein A/G contamination, we strongly recommend the use of EasyBlot Kit anti-Mouse IgG (Optimized for Prot A/G) (GTXGTX225857-01) or EasyBloker (GTX425858) to minimize protein A/G contamination or you can use anti-Mouse IgG Agarose beads (GTX27018) instead of Protein A/G.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000

Note :

WB
Use 2-5 μg of the IP antibody; do not exceed 5 μg. Completely reduce all IP samples by 100mM DTT (fresh prepared). For further detail, please refer to the protocol.
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.05M Tris, 0.15M NaCl, 1%BSA, 40% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.03 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Target Isotype

IgG

Purification

Purified by antigen-affinity chromatography.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_10728926

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

EasyBlot anti Mouse IgG (HRP). GTX221667-01

GTX221667-01 WB Image

Western Blot analysis of native mouse IgG (left) or denatured mouse IgG (right) using EasyBlot anti Mouse IgG (HRP) antibody.

EasyBlot anti Mouse IgG (HRP). GTX221667-01

GTX221667-01 WB Image

EasyBlot anti-Mouse IgG (HRP) (GTX221667-01) was used as a secondary reagent for immunoprecipitation analysis. 293T lysates were immunoprecipitated using 5 ug of normal mouse IgG or ELP3 antibody [GT11811] (GTX631395)

REFERENCE

Application Reference

Li M et al. Cell Death Dis 2024; 15 (6) : 409 Glucose deprivation triggers DCAF1-mediated inactivation of Rheb-mTORC1 and promotes cancer cell survival.
Zhang X et al. Autophagy 2024; 20 (6) : 1314-1334 Targeting cancer-associated fibroblast autophagy renders pancreatic cancer eradicable with immunochemotherapy by inhibiting adaptive immune resistance.
M Yang et al. Biol Direct 2024; 19 (1) : 46 The deubiquitinating protein OTUD6B promotes lung adenocarcinoma progression by stabilizing RIPK1
Sz?ber Z et al. Front Mol Neurosci 2024; 17 : 1359067 Neuroligin-1 dependent phosphotyrosine signaling in excitatory synapse differentiation.
F Ye et al. Cell Rep 2024; 43 (4) : 114002 Endocytic activation and exosomal secretion of matriptase stimulate the second wave of EGF signaling to promote skin and breast cancer invasion
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REVIEW

EasyBlot anti Mouse IgG (HRP) Cat. No. GTX221667-01

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
EasyBlot anti Mouse IgG (HRP). GTX221667-01
Date : Anonymous submitted on 07-Feb-2018
Score :
Application Tested : WB
Sample : HSC3 cells
Lane Description : Immunoprecipitation of p53 protein. Input, HSC3 cells (Lane 1). HSC3 cells subjected to immunoprecipitation using p53 antibody (Lane 2) or protein G Sepharose (Lane 3). The precipitated protein was detected by RUVBL1 antibody. EasyBlot anti Mouse IgG (HRP) (GTX221667-01) was used as a secondary reagent in Western blot and can avoid the interference of IgG heavy chains and light chains.
Submit a Review
Application Reference
Li M et al. Cell Death Dis 2024; 15 (6):409 Glucose deprivation triggers DCAF1-mediated inactivation of Rheb-mTORC1 and promotes cancer cell survival.
Zhang X et al. Autophagy 2024; 20 (6):1314-1334 Targeting cancer-associated fibroblast autophagy renders pancreatic cancer eradicable with immunochemotherapy by inhibiting adaptive immune resistance.
M Yang et al. Biol Direct 2024; 19 (1):46 The deubiquitinating protein OTUD6B promotes lung adenocarcinoma progression by stabilizing RIPK1
Sz?ber Z et al. Front Mol Neurosci 2024; 17:1359067 Neuroligin-1 dependent phosphotyrosine signaling in excitatory synapse differentiation.
F Ye et al. Cell Rep 2024; 43 (4):114002 Endocytic activation and exosomal secretion of matriptase stimulate the second wave of EGF signaling to promote skin and breast cancer invasion
Oudart M et al. Cell Rep 2023; 42 (5):112456 The ribosome-associated protein RACK1 represses Kir4.1 translation in astrocytes and influences neuronal activity.
June Seok Heo et al. Sci Rep 2022; 12 (1):2776 Human adipose mesenchymal stem cells modulate inflammation and angiogenesis through exosomes.
Lee SH et al. Cell Death Dis 2021; 12 (11):997 p62/SQSTM1-induced caspase-8 aggresomes are essential for ionizing radiation-mediated apoptosis.
Kim BM et al. J Nutr Biochem 2021; 95:108769 PAR2 promotes high-fat diet-induced hepatic steatosis by inhibiting AMPK-mediated autophagy.
Luanpitpong S et al. Stem Cells 2021; Metabolic sensor O-GlcNAcylation regulates megakaryopoiesis and thrombopoiesis through c-Myc stabilization and integrin perturbation.
Niklander S et al. FEBS Open Bio 2020; 10 (12):2740-2749 ROCK inhibition modulates the senescence-associated secretory phenotype (SASP) in oral keratinocytes.
Deville SS et al. Int J Mol Sci 2020; 21 (19) c-Abl Tyrosine Kinase Is Regulated Downstream of the Cytoskeletal Protein Synemin in Head and Neck Squamous Cell Carcinoma Radioresistance and DNA Repair.
Letellier M et al. Elife 2020; 9 Optogenetic control of excitatory post-synaptic differentiation through neuroligin-1 tyrosine phosphorylation.
Bieging-Rolett KT et al. Mol Cell 2020; 80 (3):452-469.e9 Zmat3 Is a Key Splicing Regulator in the p53 Tumor Suppression Program.
Tao F et al. Ann Neurol 2019; 85 (3):316-330 Variation in SIPA1L2 is correlated with phenotype modification in Charcot- Marie- Tooth disease type 1A.
Lin MW et al. Neurotherapeutics 2019; (Epub) Galantamine Inhibits Aβ1-42-Induced Neurotoxicity by Enhancing α7nAChR Expression as a Cargo Carrier for LC3 Binding and Aβ1-42 Engulfment During Autophagic Degradation.
Huang X et al. Cancer Immunol Res 2019; (Epub) USP22 deubiquitinates CD274 to suppress anti-cancer immunity.
McComb S et al. Sci Adv 2019; 5 (7):eaau9433 Efficient apoptosis requires feedback amplification of upstream apoptotic signals by effector caspase-3 or -7.
Cui X et al. Viruses 2019; 11 (6) Cellular Interleukin Enhancer-Binding Factor 2, ILF2, Inhibits Japanese Encephalitis Virus Replication In Vitro.
Porter AP et al. J Cell Sci 2019; 132 (14) The interaction between CASK and the tumour suppressor Dlg1 regulates mitotic spindle orientation in mammalian epithelia.
Nayak A et al. Cell Rep 2019; 27 (9):2725-2736.e4 Regulation of SETD7 Methyltransferase by SENP3 Is Crucial for Sarcomere Organization and Cachexia.
Huang X et al. Autophagy 2019;:1月22日 The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance.
Letellier M et al. Nat Commun 2018; 9 (1):3979 A unique intracellular tyrosine in neuroligin-1 regulates AMPA receptor recruitment during synapse differentiation and potentiation.
Su Z et al. Mol Cancer Ther 2018; (Epub) Anti-MET VHH Pool Overcomes MET-Targeted Cancer Therapeutic Resistance.
Mathieu Letellier et al. Nature Communication 2018; 9:3979 A unique intracellular tyrosine in neuroligin-1 regulates AMPA receptor recruitment during synapse differentiation and potentiation.
Chen J et al. Mol Plant Pathol 2018; (Epub) A novel Meloidogyne graminicola effector, MgMO237, interacts with multiple host defense-related proteins to manipulate plant basal immunity and promote parasitism.
Huang SC et al. EMBO Mol Med 2017; (Epub) TGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB.
Shinohara K et al. Hypertension 2017; (Epub) Selective Deletion of Renin-b in the Brain Alters Drinking and Metabolism.
Nashida T et al. Odontology 2017; (Epub) Presence of BPIFB1 in saliva from non-obese diabetic mice.
Viant C et al. J Exp Med 2017; (Epub) Cell cycle progression dictates the requirement for BCL2 in natural killer cell survival.
Fan W et al. Sci Rep 2016; 6:33698 TRIM52 inhibits Japanese Encephalitis Virus replication by degrading the viral NS2A.
Barel M et al. Cell Microbiol 2016; (Epub) Host glycosylation pathways and the unfolded protein response contribute to the infection by Francisella.
Xing C et al. Cancer Res 2016; 76 (1):83-95 Ubiquitin-Specific Protease 4-Mediated Deubiquitination and Stabilization of PRL-3 Is Required for Potentiating Colorectal Oncogenesis.
Wang B et al. Nat Commun 2015; 6:8704 POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation.
Shang ZF et al. Int J Biol Sci 2015; 11 (9):1026-35 DNA-PKcs Negatively Regulates Cyclin B1 Protein Stability through Facilitating Its Ubiquitination Mediated by Cdh1-APC/C Pathway.
Wu MH et al. Oncogene 2014; (Epub) MCT-1 expression and PTEN deficiency synergistically promote neoplastic multinucleation through the Src/p190B signaling activation.
SDS
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 199
$ 89