ELISA: Use at an assay dependent dilution. IHC-Fr: Use at a concentration of 3-10 μg/ml. Optimal dilutions/concentrations should be determined by the end user.
Sensitivity has been determined by ELISA. Less than 1ng of antibody could be detected after binding to Endomorphin 2 which is conjugated to titer plate:Endomorphin 2 = 100Endomorphin 1 = 70Leu5-Enkephalin = <0.01Met5-Enkephalin = <0.03beta-Endorphin = <0.01
Restore with 50μl of dH2O containing 0.1% sodium azide or other preservative
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Synthetic peptide conjugated to BSA.
Immunogen affinity purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Endomorphin 1 &2
Recently, endogenous agonists for the mu-opiate receptor, were first isolated from bovine brain [Nature,386, 499 (1997)]. These are endomorphin-1; Tyr-Pro-Trp-Phe-NH2, and endomorphin-2; Tyr-Pro-Phe-Phe-NH2. The isolation of relatively large amounts of endomorphin-1 and endomorphin-2 from human brain cortex has also reported [Peptides,18, 1635 (1997)].The immunoreactivity for endomorphin was also detected in rat brain near the mu-opiate receptor containing neurons, but the molecular form of the rat peptide has not yet been elucidated [Peptides,18, 1641 (1997)]. These observations may be considered to indicate that endomorphin-1 and 2 are common mu-opiate agonists in mammals. In addition to the originally discovered mu-opiate agonistic function, these peptides have been reported to show hypotensive activity and Ca2+ channel current inhibitory activity [Biochem. Biophys. Res. Commun.,235, 567 (1997)]. Endomorphins would be useful for the study of pain perception together with some other biological functions in the body.
GTX10290 IHC Image
Endomorphin-1 and 2 staining in rat paraventricular thalamic nucleus.