FACS analysis of HepG2 cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

ICC/IF analysis of HUVEC cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

ICC/IF analysis of Hela cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

FACS analysis of 293T cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

ICC/IF analysis of PC12 cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

FACS analysis of 3T3 cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

WB analysis of 25 ug of PC12 cell lysate using GTX22786 Endothelin 1 antibody [TR.ET.48.5].
Dilution : 1:500