Enterovirus 71 VP1 antibody

Cat. No. GTX132338

Reference 13

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Enterovirus 71
Package	100 μl ($399), 25 μl ($169)

See all Enterovirus 71 VP1 products

View product citations for antibody GTX132338 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Positive Control

EV71 C2 infected RD , EV71 B4 infected RD , CVA16 infected RD

Product Note

This antibody was raised against Enterovirus 71 VP1. It recognizes Enterovirus 71 VP1 signal and shows cross-react with Coxsackievirus A16 VP1. However, it does not cross-react with Enterovirus D68 VP1.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Enterovirus 71

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Enterovirus 71 VP1 (EV71 virus (strain USA/BrCr/1970)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886616

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Enterovirus 71 Capsid protein VP1 , Enterovirus 71 VP1 , EV71 Capsid protein VP1 , EV71 VP1

Research Area

DATA IMAGES

GTX132338 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody (GTX132338) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX132338 IHC-P Image

EV71 VP1 antibody detects EV71 VP1 protein in EV71 infected mouse skeletal muscle by immunohistochemical analysis. Sample: Paraffin-embedded EV71 infected mouse skeletal muscle. EV71 VP1 antibody [GTX132338] diluted at 1:500
Antigen Retrieval: Citrate buffer

GTX132338 WB Image

Non-infected (–) and infected RD whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody (GTX132338) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX132338 ICC/IF Image

EV71 VP1 antibody detects EV71 VP1 protein by immunofluorescent analysis. Sample: RD cells infected with EV71 were fixed in 4% paraformaldehyde. Green: EV71 VP1 protein stained by EV71 VP1 antibody (GTX132338) diluted at 1:100. Blue: DAPI staining. Scale bar = 500 μm.