EpCAM antibody [GT25512]

Cat. No. GTX635967
Cat. No.  GTX635967
Orthogonal
Anti-EpCAM antibody [GT25512] used in Flow cytometry (FCM). GTX635967
GTX635967 FCM Image
Orthogonal

HCT116 (EpCAM positive), HeLa (EpCAM negative)

EpCAM antibody[GT25512] (GTX635967) detects EpCAM by flow cytometry analysis.
Sample: HeLa and HCT-116
Blue: Mouse IgG isotype control.
Red: EpCAM antibody[GT25512] (GTX635967) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.
HCT116 (EpCAM positive), HeLa (EpCAM negative)

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Anti-EpCAM antibody [GT25512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635967
GTX635967 ICC/IF Image
Orthogonal

EpCAM antibody [GT25512] detects EpCAM protein at cell membrane by immunofluorescent analysis.
Sample: HCT116 and HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: EpCAM stained by EpCAM antibody [GT25512] (GTX635967) diluted at 1:500.

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Anti-EpCAM antibody [GT25512] used in IHC (Paraffin sections) (IHC-P). GTX635967
GTX635967 IHC-P Image

EpCAM antibody [GT25512] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded MDA-MB-231 xenograft.
EpCAM stained by EpCAM antibody [GT25512] (GTX635967) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Anti-EpCAM antibody [GT25512] used in Western Blot (WB). GTX635967
GTX635967 WB Image
Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with EpCAM antibody [GT25512] (GTX635967) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX2131

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Anti-EpCAM antibody [GT25512] used in Flow cytometry (FCM). GTX635967
Anti-EpCAM antibody [GT25512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635967
Anti-EpCAM antibody [GT25512] used in IHC (Paraffin sections) (IHC-P). GTX635967
Anti-EpCAM antibody [GT25512] used in Western Blot (WB). GTX635967
  • Host
    Mouse
  • Clonality
    Monoclonal
  • Clone Name
    GT25512
  • Isotype
    IgG2a
  • Applications
    WB ICC/IF IHC-P FCM
  • Reactivity
    Human
Summary
EpCAM antibody recognizes epithelial cell adhesion molecule (EpCAM), a transmembrane glycoprotein with a predicted molecular weight of ~35 kDa. EpCAM is upregulated in epithelial cells during epithelial–mesenchymal transition (EMT) and is used as a diagnostic marker of many carcinomas and circulating tumor cells. Detection of EpCAM by immunohistochemistry is important for the diagnosis of certain epithelial malignancies.
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