Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using Ep-CAM (GTX113091) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

EpCAM antibody [N3C3] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
EpCAM stained by EpCAM antibody [N3C3] (GTX113091) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EpCAM antibody [N3C3] (GTX113091) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

EpCAM antibody [N3C3] detects EpCAM protein at cell membrane and cell junction by immunofluorescent analysis.
Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.
Green: EpCAM stained by EpCAM antibody [N3C3] (GTX113091) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

The data was published in the journal Oncotarget in 2016. PMID: 27564110