GeneTex
United States (US)

EphB2 antibody

Cat No. GTX10616

Host Goat
Clonality Polyclonal
Isotype IgG
Application Dot, ELISA
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
Dot Assay dependent
ELISA Assay dependent

Note :

ELISA
using protease-digested, human and animal tissue sections

Not tested in other applications.

Specificity/Sensitivity

In immunoblotting, the antibody exhibits ~5% cross-reactivity with recombinant rat EphB1 and EphA5, recombinant mouse EphA8, EphB6, EphA3, EphA4, EphA7, EphA2, EphB3, and recombinant human EphA1.
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

Purified recombinant mouse EphB2 extracellular domain expressed in NSO cells.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eph Receptor B2,Cek5,Drt,Eteck,Erk,Hek5,Nuk,Prkm5,Qek5,Sek3,Tyro5,Ephb2

Background

EphB2, also known as Cek5, Hek5, Tyro5, Se3, Nuk, Erk, Qek2, and Drt, is a member of the Eph receptor family, which binds members of the ephrin ligand family. Two classes of receptors exist, designated A and B, that have an extracellular domain made up of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosines, which are the major autophosphorylation sites, along with a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxyl terminus, which includes one conserved tyrosine. The recombinant mouse EphB2 used for the testing of this antibody consists of the extracellular domain of mouse EphB2 (amino acids 1-548)1 fused by means of a polypeptide linker to the Fc portion of human IgG1 that is histidine-tagged at the C-terminus. N-terminal sequencing indicates that recombinant mouse EphB2 has Val 27 at the amino terminus. The calculated molecular mass of the reduced protein is approximately 85.3 kDa, but as a result of glycosylation, recombinantEphB2/Fc migrates as a 100-110 kDa protein under reducing conditions in SDS-PAGE. EphB2 binds to ephrin-B1, ephrin-B2, and ephrin-B3. Human and mouse EphB3 extracellular domains share approximately 99% homology. Ligand recognition and binding leads to activation of intrinsic kinase activity. Only membrane-bound or Fc-clustered ligands have been shown to activate the receptor in vitro. Soluble monomeric ligands can bind the receptor, but do not induce receptor autophosphorylation and activation. The ephrin ligands and Eph receptors display reciprocal expression in vivo. Developing and adult neural tissue express nearly all of the Eph receptors and ephrin ligands. Ephs and ephrins play a significant role in angiogenesis.

Research Area

SDS
PBS.pdf
Package List Price ($)
$ 329