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Estrogen Receptor beta antibody [14C8]

Anti-Estrogen Receptor beta antibody [14C8] used in Western Blot (WB). GTX70174
Anti-Estrogen Receptor beta antibody [14C8] used in Western Blot (WB). GTX70174
Anti-Estrogen Receptor beta antibody [14C8] used in Immunohistochemistry (IHC). GTX70174
Anti-Estrogen Receptor beta antibody [14C8] used in IHC (Paraffin sections) (IHC-P). GTX70174

Cat No. GTX70174

Host

Mouse

Clonality

Monoclonal

Clone Name

14C8

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, Dot, ChIP assay, IHC

Reactivity

Human, Mouse, Monkey
Package
100 μl ($329)

APPLICATION

Application Note

For IHC, a recommended protocol is provided. For WB: Use at a concentration of 1-5 μg/ml. For ChIP: Use at an dependent assay. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

59 kDa. ( Note )

Positive Control

Myc-DDK-tagged ESR2-transfected 293T

Product Note

Strong Erb staining in epithelial cell nuclei. Occasional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also successfully detected ERb in colon and ovarian tumors using the same antibody.
Knockdown/Knockout validation was supported by references (PMID:27323858)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 1-153 of human ER-beta expressed in E. coli.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_370367

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

estrogen receptor 2 , ER-BETA , ESR-BETA , ESRB , ESTRB , Erb , NR3A2 , ODG8

Cellular Localization

Nucleus

Background

This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Estrogen Receptor beta antibody [14C8] used in Western Blot (WB). GTX70174

GTX70174 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [14C8] (GTX70174) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Estrogen Receptor beta antibody [14C8] used in Western Blot (WB). GTX70174

GTX70174 WB Image

Various tissue extracts (20 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [14C8] (GTX70174) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Estrogen Receptor beta antibody [14C8] used in Immunohistochemistry (IHC). GTX70174

GTX70174 IHC Image

B. Infiltrating lobular carcinoma of the breast.

Anti-Estrogen Receptor beta antibody [14C8] used in IHC (Paraffin sections) (IHC-P). GTX70174

GTX70174 IHC-P Image

Estrogen Receptor beta antibody [14C8] detects Estrogen Receptor beta protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Estrogen Receptor beta stained by Estrogen Receptor beta antibody [14C8] (GTX70174) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

Estrogen Receptor beta antibody [14C8] Cat No. GTX70174

Rating ( Average 4.9 based on 8 users reviews)
Application
Western Blot(WB) ( Average 5 based on 5 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cancer Med in 2013.PMID: 23930207
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Reprod Biol Endocrinol in 2012.PMID: 22520060
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Breast Cancer Res in 2012.PMID: 23158001
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Breast Cancer Res in 2012.PMID: 23158001
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Breast Cancer Res in 2012.PMID: 23158001
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Breast Cancer Res in 2012.PMID: 23158001
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Breast Cancer Res in 2012.PMID: 23158001
Date : Anonymous submitted on 13-Feb-2019
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : Cells differentiated from neural stem cells
Blocking : 10% goat serum in PBS, 25°C, 0.5Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5% Tritonx-100 in PBS
Primary Antibody : 1:500, 4°C, 16Hr
Application Reference
SDS
PBS.pdf