GeneTex
  • Country / Location Selection

United States (US)

Exonuclease 1 antibody

Anti-Exonuclease 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109891
Anti-Exonuclease 1 antibody used in Western Blot (WB). GTX109891
Anti-Exonuclease 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109891
Anti-Exonuclease 1 antibody used in Western Blot (WB). GTX109891

Cat No. GTX109891

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

Positive Control

Jurkat , NCI-H929 , Mouse thymus
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Exonuclease 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

exonuclease 1 , HEX1 , hExoI

Cellular Localization

Nucleus

Background

This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Exonuclease 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109891

GTX109891 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Exonuclease 1(GTX109891) antibody at 1:500 dilution.

Anti-Exonuclease 1 antibody used in Western Blot (WB). GTX109891

GTX109891 WB Image

Sample (50 µg of whole cell lysate)
A: mouse thymus
7.5% SDS PAGE
GTX109891 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Exonuclease 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109891

GTX109891 ICC/IF Image

Exonuclease 1 antibody detects Exonuclease 1 protein at nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Exonuclease 1 protein stained by Exonuclease 1 antibody (GTX109891) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-Exonuclease 1 antibody used in Western Blot (WB). GTX109891

GTX109891 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Exonuclease 1 antibody (GTX109891) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
There are currently no reviews for Exonuclease 1 antibody (GTX109891). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169