Ezrin antibody [N2C2], Internal

Ezrin antibody [N2C2], Internal detects Ezrin protein at cytoplasm and membrane in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Ezrin antibody [N2C2], Internal detects Ezrin protein at cytoplasm by immunofluorescent analysis.
Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.
Green: Ezrin protein stained by Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Ezrin(GTX111709) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Ezrin antibody [N2C2], Internal detects Ezrin protein at cell membrane by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ezrin stained by Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Ezrin(GTX111709) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Ezrin antibody [N2C2], Internal detects Ezrin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
Ezrin stained by Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Ezrin antibody [N2C2], Internal detects Ezrin protein on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
Ezrin antibody [N2C2], Internal (GTX111709) dilution: 1:200.

Ezrin antibody [N2C2], Internal detects Ezrin protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Ezrin stained by Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC