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Ezrin antibody [N2C2], Internal

Anti-Ezrin antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111709
Anti-Ezrin antibody [N2C2], Internal used in Western Blot (WB). GTX111709
Anti-Ezrin antibody [N2C2], Internal used in Western Blot (WB). GTX111709
Anti-Ezrin antibody [N2C2], Internal used in IHC (Whole mount) (IHC-Wm). GTX111709
Anti-Ezrin antibody [N2C2], Internal used in IHC (Whole mount) (IHC-Wm). GTX111709
Anti-Ezrin antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111709
Anti-Ezrin antibody [N2C2], Internal used in Western Blot (WB). GTX111709
Anti-Ezrin antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111709
Anti-Ezrin antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111709
Anti-Ezrin antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111709
Anti-Ezrin antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111709

Cat. No. GTX111709

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Ezrin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Ezrin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170466

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ezrin , CVIL , CVL , HEL-S-105 , VIL2

Cellular Localization

Apical cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell projection , microvillus membrane; Peripheral membrane protein; Cytoplasmic side , ruffle membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , cell cortex , cytosk

Background

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Ezrin antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111709

GTX111709 IHC-P Image

Ezrin antibody [N2C2], Internal detects Ezrin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
Ezrin stained by Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ezrin antibody [N2C2], Internal used in Western Blot (WB). GTX111709

GTX111709 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Ezrin antibody [N2C2], Internal used in Western Blot (WB). GTX111709

GTX111709 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Ezrin antibody [N2C2], Internal used in IHC (Whole mount) (IHC-Wm). GTX111709

GTX111709 IHC-Wm Image

Ezrin antibody [N2C2], Internal detects Ezrin protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Ezrin stained by Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-Ezrin antibody [N2C2], Internal used in IHC (Whole mount) (IHC-Wm). GTX111709

GTX111709 IHC-Wm Image

Ezrin antibody [N2C2], Internal detects Ezrin protein on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
Ezrin antibody [N2C2], Internal (GTX111709) dilution: 1:200.
Anti-Ezrin antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111709

GTX111709 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Ezrin(GTX111709) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Ezrin antibody [N2C2], Internal used in Western Blot (WB). GTX111709

GTX111709 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Ezrin antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111709

GTX111709 ICC/IF Image

Ezrin antibody [N2C2], Internal detects Ezrin protein at cell membrane by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ezrin stained by Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Ezrin antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111709

GTX111709 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Ezrin(GTX111709) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Ezrin antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111709

GTX111709 ICC/IF Image

Ezrin antibody [N2C2], Internal detects Ezrin protein at cytoplasm by immunofluorescent analysis.
Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.
Green: Ezrin protein stained by Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-Ezrin antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111709

GTX111709 IHC-P Image

Ezrin antibody [N2C2], Internal detects Ezrin protein at cytoplasm and membrane in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Liang Y et al. Int J Mol Sci 2022; 23 (16) Aberrant Retinal Pigment Epithelial Cells Derived from Induced Pluripotent Stem Cells of a Retinitis Pigmentosa Patient with the PRPF6 Mutation.
Wang SA et al. Cell Death Differ 2021; (Epub) USP24 promotes drug resistance during cancer therapy.
Application : WB
Reactivity : Human
Chang SJ et al. Arch Biochem Biophys 2018; 647 : 10-32 Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma.
Application : WB
Reactivity : Human
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB
Reactivity : Human
May EW et al. Pharmacol Res 2014; (Epub) identification of up- and down-regulated proteins in *-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of *-associated resistance.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169