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Ezrin antibody [N2C2], Internal

Anti-Ezrin antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111709
Anti-Ezrin antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111709
Anti-Ezrin antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111709
Anti-Ezrin antibody [N2C2], Internal used in Western Blot (WB). GTX111709
Anti-Ezrin antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111709
Anti-Ezrin antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111709

Cat No. GTX111709

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

293T , A431 , HeLa

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Ezrin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ezrin,CVIL,CVL,HEL-S-105,VIL2

Cellular Localization

Apical cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell projection , microvillus membrane; Peripheral membrane protein; Cytoplasmic side , ruffle membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , cell cortex , cytosk

Background

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Ezrin antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111709

GTX111709 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Ezrin(GTX111709) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Ezrin antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111709

GTX111709 ICC/IF Image

Ezrin antibody [N2C2], Internal detects Ezrin protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ezrin protein stained by Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-Ezrin antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111709

GTX111709 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Ezrin(GTX111709) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Ezrin antibody [N2C2], Internal used in Western Blot (WB). GTX111709

GTX111709 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
7.5% SDS PAGE
GTX111709 diluted at 1:10000

Anti-Ezrin antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111709

GTX111709 IHC-P Image

Ezrin antibody [N2C2], Internal detects Ezrin protein at cytoplasm and membrane in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ezrin antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111709

GTX111709 ICC/IF Image

Ezrin antibody [N2C2], Internal detects Ezrin protein at cytoplasm by immunofluorescent analysis.
Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.
Green: Ezrin protein stained by Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:1000.
Blue: Hoechst 33342 staining.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169