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FACL4 antibody [C3], C-term

Anti-FACL4 antibody [C3], C-term used in Western Blot (WB). GTX100260
Anti-FACL4 antibody [C3], C-term used in Western Blot (WB). GTX100260
Anti-FACL4 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100260
Anti-FACL4 antibody [C3], C-term used in Immunoprecipitation (IP). GTX100260
Anti-FACL4 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100260
Anti-FACL4 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100260
Anti-FACL4 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100260
Anti-FACL4 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100260

Cat No. GTX100260

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:1000-1:5000
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

MCF-7 , MDA-MB-231 , NIH-3T3 , JC , BCL-1

Predict Reactivity

Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FACL4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

acyl-CoA synthetase long chain family member 4,ACS4,FACL4,LACS4,MRX63,MRX68

Cellular Localization

Mitochondrion outer membrane; Single-pass type III membrane protein , Peroxisome membrane; Single-pass type III membrane protein , Microsome membrane; Single-pass type III membrane protein , Endoplasmic reticulum membrane; Single-pass type III membrane pr

Background

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-FACL4 antibody [C3], C-term used in Western Blot (WB). GTX100260

GTX100260 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX100260 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FACL4 antibody [C3], C-term used in Western Blot (WB). GTX100260

GTX100260 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [C3], C-term (GTX100260) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FACL4 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100260

GTX100260 IHC-P Image

FACL4 antibody [C3], C-term detects FACL4 protein at cytosol on mouse lymph node by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
FACL4 antibody [C3], C-term (GTX100260) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FACL4 antibody [C3], C-term used in Immunoprecipitation (IP). GTX100260

GTX100260 IP Image

Immunoprecipitation of FACL4 protein from HeLa whole cell extracts using 5 µg of FACL4 antibody [C3], C-term (GTX100260).
Western blot analysis was performed using FACL4 antibody [C3], C-term (GTX100260).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.

Anti-FACL4 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100260

GTX100260 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using FACL4(GTX100260) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-FACL4 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100260

GTX100260 IHC-P Image

FACL4 antibody [C3], C-term detects FACL4 protein at cytosol on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
FACL4 antibody [C3], C-term (GTX100260) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FACL4 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100260

GTX100260 ICC/IF Image

FACL4 antibody [C3], C-term detects FACL4 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FACL4 protein stained by FACL4 antibody [C3], C-term (GTX100260) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-FACL4 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100260

GTX100260 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using FACL4(GTX100260) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169