Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using FADS1(GTX114528) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

FADS1 antibody detects FADS1 protein at mitochondria by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: FADS1 protein stained by FADS1 antibody (GTX114528) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX114528 diluted at 1:1000