GeneTex
United States (US)

FAK (phospho Tyr861) antibody

Orthogonal
GTX24804_619_ICC-IF_19011519_719.jpg
GTX24804_1728_WB_19011519_270.jpg

Cat No. GTX24804

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, IHC
Reactivity Human, Mouse, Rat, Zebrafish, Dog, Hamster, Chicken, Amphibians, Xenopus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1 μg/ml
IHC-P Assay dependent
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

119 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 50% glycerol, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains tyrosine 861. The sequence is conserved in mouse, rat, chicken and frog.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FADK,FAK,FAK1,FRNK,PPP1R71,p125FAK,pp125FAK

Cellular Localization

Cell cortex,Cell junction,Cell membrane,Centrosome,Cytoplasm,Cytoskeleton,Focal adhesion,Microtubule organizing center,Nucleus

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]

Database

Research Area

DATA IMAGES
GTX24804_619_ICC-IF_19011519_719.jpg

GTX24804 ICC/IF Image

ICC/IF analysis of A549 cells using GTX24804 FAK (phospho Tyr861) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1 µg/ml in 1% BSA incubated for 3 hours at room temperature

GTX24804_1728_WB_19011519_270.jpg

GTX24804 WB Image

Orthogonal

WB (peptide competition) analysis of 3T3-L1 cells stimulated with 50 ng/ml LIF for 15 minutes (Lane 2-4) using GTX24804 FAK (phospho Tyr861) antibody prior incubated with the phosphopeptide immunogen (Lane 1), or genertic phosphopeptide (Lane 2), the non-phosphopeptide corresponding to generic phosphoserine-containing peptide (Lane 3), or no peptide (Lane 4).. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

Package List Price ($)
$ 319