FAK (phospho Tyr861) antibody

Cat. No. GTX24804

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP, IHC
Reactivity	Human, Mouse, Rat, Zebrafish, Dog, Hamster, Chicken, Amphibians, Xenopus
Package	50 μl ($399)

See all FAK products

Datasheet
Document

View product citations for antibody GTX24804 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000
ICC/IF	1 μg/ml
IHC-P	Assay dependent
IP	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW

119 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains tyrosine 861. The sequence is conserved in mouse, rat, chicken and frog.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine kinase 2 , FADK , FAK , FAK1 , FRNK , PPP1R71 , p125FAK , pp125FAK

Cellular Localization

Cytoplasm, cytoskeleton,Nucleus

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]

Database

Gene ID: 5747 PTK2
UniProt: Q05397 PTK2

Research Area

DATA IMAGES

GTX24804 WB Image

WB (peptide competition) analysis of 3T3-L1 cells stimulated with 50 ng/ml LIF for 15 minutes (Lane 2-4) using GTX24804 FAK (phospho Tyr861) antibody prior incubated with the phosphopeptide immunogen (Lane 1), or genertic phosphopeptide (Lane 2), the non-phosphopeptide corresponding to generic phosphoserine-containing peptide (Lane 3), or no peptide (Lane 4).. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

GTX24804 ICC/IF Image

ICC/IF analysis of A549 cells using GTX24804 FAK (phospho Tyr861) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1 μg/ml in 1% BSA incubated for 3 hours at room temperature

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

50 μl

$ 399

Qty