Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with FDFT1 antibody [N1C3] (GTX108885) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using Squalene synthetase(SQS)(GTX108885) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FDFT1 antibody [N1C3] (GTX108885) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.