GeneTex
United States (US)

FGFR1 antibody

GTX10646_20170605_IHC-P_19011506_247.jpg
GTX10646_20170605_IHC-P_1_19011506_179.jpg
GTX10646_20170605_ICCIF_19011506_712.jpg

Cat No. GTX10646

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:400
ICC/IF Assay dependent
IHC-P 1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Specificity/Sensitivity

Anti-Fibroblast Growth Factor Receptor-1 (FGFR-1) is developed in rabbit using a synthetic peptide Glu-AlaLeu-Glu-Glu-Arg-Pro-Ala-Val-Met-Thr-Ser-Pro-Leu-Lys conjugated to KLH with glutaraldehyde as immunogen. The peptide corresponds to amino acid residues 360-373 of the extracellular region of human FGFR-1 with a C-terminally added lysine.Staining of the product is specifically inhibited with the FGFR-1 immunizing peptide. No reaction with human FGFR-2 and FGFR-3 is detected.
PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 1% bovine serum albumin, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to amino acid residues 360-373 of the extracellular region of human FGFR-1 conjugated to KLH with glutaraldehyde, and with a C-terminally added lysine.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fibroblast Growth Factor Receptor 1,Bfgfr,Cd331,Cek,Eccl,Fgfbr,Fgfr-1,Flg,Flt-2,Flt2,Hbgfr,Hh2,Hrtfds,Kal2,N-Sam,Ogd,Bfgf-R-1,Fgfr1

Background

Fibroblast growth factors (FGFs) are members of a large family of structurally related polypeptides (17-38 kDa) that are potent physiological regulators of growth and differentiation of a wide variety of cells of mesodermal, ectodermal and endodermal origin. FGFs are substantially involved in normal development, wound healing and repair, angiogenesis, a variety of neurotrophic activities, in hematopoiesis as well as in tissue remodeling and maintenance. They also have been implicated in pathological conditions such as tumorigenesis and metastasis. To date, the FGF family consists of at least 23 members designated FGF1 through FGF23. Four genes encoding for high affinity cell surface FGF receptors (FGFRs) have been identified: FGFR1 [flg-1(fms-like gene 1)]; FGFR2 [bek (bacterial expressed kinase gene product)]; FGFR3 (cek-2), and FGFR4. Multiple additional variants (isoforms) arising by alternative splicing have been reported: soluble, secreted, or possibly cleaved forms of FGFR1 and FGFR2 have also been found in body fluids or were artificially constructed, [e.g. a soluble FGF-binding protein containing the extracellular region of FGFR1 and the secreted form of placental alkaline phosphatase (FRAP1)]. FGFRs are members of the tyrosine kinase family of growth factor receptors. They are glycosylated 110- 150 kDa proteins that are constructed of an extracellular ligand binding region with either two (alpha type) or typically three (alpha type) immunoglbulin (Ig)-like domains and an eight amino acid acidic box, a transmembrane region, and a cytoplasmic split tyrosine kinase domain that is activated following ligand binding and receptor dimerization. The ligand binding site of FGFRs is confined to the extracellular Ig-like domains 2 and 3.FGFRs exhibit overlapping recognition and redundant specificity. One receptor type may bind with a similar affinity several of the FGFs. Also one FGF type may bind similarly to several distinct receptors. This accounts for the rather identical effects of different FGF ligands on common cell types. FGFis binding to cellular FGFRs depend on or is markedly facilitated by the low-affinity interaction of FGF with the polysaccharide component of the cell surface or extracellular matrix heparan sulfate proteoglycans (HSPG). For example, perlecan, a basement membrane HSPG, promotes high affinity binding of FGF2 in vitro and angiogenesis in vivo. Signal transduction by FGFRs requires dimerization or oligomerization and autophosphorylation of the receptors through their tyrosine kinase domain. Subsequent association with cytoplasmic signaling molecules leads to DNA synthesis or differentiation. The signaling and biological responses elicited by distinct FGFRs substantially differ and are dictated by the intracellular domain. At the mRNA level, FGFR1 is highly expressed in developing human tissues including the brain (preferentially in neurons), vascular basement membranes, skin, and bone growth plates. It may be found in most anchorage dependent cells on their membrane and also may be localized around and in nuclei. Pfeiffer syndrome, as well as other disorders of human skeletal development, is the result of a mutation in the extracellular domain of FGFR1.

Database

Research Area

DATA IMAGES
GTX10646_20170605_IHC-P_19011506_247.jpg

GTX10646 IHC-P Image

IHC-P analysis of human umbilical cord sections using GTX10646 FGFR1 antibody at 5 μg/mL.

GTX10646_20170605_IHC-P_1_19011506_179.jpg

GTX10646 IHC-P Image

IHC-P analysis of human umbilical cord section using GTX10646 FGFR1 antibody at 5 μg/mL.

GTX10646_20170605_ICCIF_19011506_712.jpg

GTX10646 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX10646 FGFR1 antibody at 1:100(red) with DAPI(blue). Cells were fixed and permeabilized methanol followed by methanol:acetone.

Package List Price ($)
$ 319