GeneTex

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United States (US)

FITC antibody

Cat. No. GTX19490

Reference 2

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG F(ab')2

Application

 WB, ICC/IF, IHC-P, IHC-Fr, ELISA, IHC
Package
100 μg ($399)
See all FITC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200 - 1:1000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
ELISA 1:2000 - 1:8000
IHC Assay dependent
Not tested in other applications.

Product Note

No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate pH7.2, 0.15M Sodium Chloride

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Fluorescein conjugated to Goat IgG

Purification

Purified by antigen-affinity chromatography.
Immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Conjugation

Unconjugated

RRID

AB_423597

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

Research Area

REFERENCE

Application Reference

Uchida T et al. Ren Fail 2017; 39 (1) : 340-349 Renoprotective effects of a dipeptidyl peptidase 4 inhibitor in a mouse model of progressive renal fibrosis.
Application : IHC-P
Gotanda Y et al. J Stroke Cerebrovasc Dis 2014; Efficient Transduction of 11 Poly-arginine Peptide in an Ischemic Lesion of Mouse Brain.
Application : ICC/IF, IHC-Fr
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399