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FITC antibody

Cat No. GTX19490

Host

Goat

Clonality

Polyclonal

Isotype

IgG F(ab')2

Application

WB, ICC/IF, IHC-P, IHC-Fr, ELISA, IHC
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:200 - 1:1000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
ELISA 1:2000 - 1:8000
IHC Assay dependent
Not tested in other applications.

Specificity/Sensitivity

No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Fluorescein conjugated to Goat IgG

Purification

Purified by antigen-affinity chromatography.
Immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Conjugation

Unconjugated

RRID

AB_423597

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

Research Area

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 339