GeneTex
United States (US)

FLT3 antibody

Cat No. GTX10654

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ELISA, Neut
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.2 μg/ml
ELISA 0.5-1.0 μg/mL
Neut Assay dependent
Not tested in other applications.

Calculated MW

113 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

recombinant human Flt-3 ligand expressed in Sf 21 cells.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fms Related Tyrosine Kinase 3,Cd135,Flk-2,Flk2,Stk1,Flt3

Background

Flt3/Flk2 Ligand (FL) is produced from a DNA sequence encoding amino acid residues 1 to 185 polypeptide of Flt3/Flk2 ligand. The 159 amino acid residue recombinant protein, generated after removal of the 26 amino acid residue signal peptide, has a predicted molecular mass of approximately 17.5 kDa. The gene for FL has been mapped to chromosomes 19q13.3 and 13q12-q13 in humans. The Flt3 (fms-like tyrosine kinase 3)/Flk2 (fetal liver kinase 2) Ligand (FL), a hematopoietic cytokine, was cloned based on its ability to bind and activate c-kit and Flt3 tyrosine kinase receptors. Fl plays a key role in hematopoiesis by stimulating proliferation and/or differentiation of various hematopoietic cell types in vitro as well as in vivo. Human FL, a type I transmembrane glycoprotein, is biologically active on the cell surface and can be cleaved to generate a soluble form that is also biologically active. Also, an alternatively spliced soluble isoform of FL has been identified in both human and mouse. Human and mouse FL share 72% amino acid sequence homology and exhibit full species cross-reactivity.FL has been shown to synergize with a wide variety of hematopoietic cytokines to stimulate the growth and differentiation of early hematopoietic progenitors. FL promotes growth of early B cell progenitor cells in combination with IL-7 and induces adhesion of the precursor B cell line Baflt3 to fibronectin by activating the fibronectin receptors VLA-4 and VLA-5 integrins. Flt3/Flk2 ligand is widely expressed in a variety of tissues. Cells known to express FL include T cell lines, a thymic stromal cell line 1, and bone marrow fibroblasts. In hematopoietic cells, the expression of Flt3/Flk2 is restricted to the population enriched for precursor/progenitor cells.

Database

SDS
PBS.pdf
Package List Price ($)
$ 319