Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Batch dependent (Please refer to the vial label for the specific concentration.)
recombinant human Flt-3 ligand expressed in Sf 21 cells.
Purified by affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
fms related tyrosine kinase 3 , CD135 , FLK-2 , FLK2 , STK1
Membrane; Single-pass type I membrane protein
Flt3/Flk2 Ligand (FL) is produced from a DNA sequence encoding amino acid residues 1 to 185 polypeptide of Flt3/Flk2 ligand. The 159 amino acid residue recombinant protein, generated after removal of the 26 amino acid residue signal peptide, has a predicted molecular mass of approximately 17.5 kDa. The gene for FL has been mapped to chromosomes 19q13.3 and 13q12-q13 in humans. The Flt3 (fms-like tyrosine kinase 3)/Flk2 (fetal liver kinase 2) Ligand (FL), a hematopoietic cytokine, was cloned based on its ability to bind and activate c-kit and Flt3 tyrosine kinase receptors. Fl plays a key role in hematopoiesis by stimulating proliferation and/or differentiation of various hematopoietic cell types in vitro as well as in vivo. Human FL, a type I transmembrane glycoprotein, is biologically active on the cell surface and can be cleaved to generate a soluble form that is also biologically active. Also, an alternatively spliced soluble isoform of FL has been identified in both human and mouse. Human and mouse FL share 72% amino acid sequence homology and exhibit full species cross-reactivity.FL has been shown to synergize with a wide variety of hematopoietic cytokines to stimulate the growth and differentiation of early hematopoietic progenitors. FL promotes growth of early B cell progenitor cells in combination with IL-7 and induces adhesion of the precursor B cell line Baflt3 to fibronectin by activating the fibronectin receptors VLA-4 and VLA-5 integrins. Flt3/Flk2 ligand is widely expressed in a variety of tissues. Cells known to express FL include T cell lines, a thymic stromal cell line 1, and bone marrow fibroblasts. In hematopoietic cells, the expression of Flt3/Flk2 is restricted to the population enriched for precursor/progenitor cells.