APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 0.5-1μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human FOXA1 protein (aa372-472) (exact sequence is proprietary)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
FOXA1 , HNF3A , TCF3A , forkhead box A1
Cellular Localization
Nucleus
Background
This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-FOXA1 antibody [FOXA1/1518] used in Western Blot (WB). GTX34739](/templates/default/js/lightbox/images/loading.gif "Anti-FOXA1 antibody [FOXA1/1518] used in Western Blot (WB). GTX34739")
|
GTX34739 WB Image
WB analysis of HeLa cell lysate using GTX34739 FOXA1 antibody [FOXA1/1518].
|
|
|
GTX34739 IHC-P Image
IHC-P analysis of rat colon tissue using GTX34739 FOXA1 antibody [FOXA1/1518].
|
|
|
GTX34739 IHC-P Image
IHC-P analysis of human prostate carcinoma tissue using GTX34739 FOXA1 antibody [FOXA1/1518].
|
|
|
GTX34739 ICC/IF Image
ICC/IF analysis of PFA-fixed MCF-7 cells using GTX34739 FOXA1 antibody [FOXA1/1518].
Green : Primary antibody
Red : Actin
|
|
|
GTX34739 FACS Image
FACS analysis of PFA-fixed MCF-7 cells using GTX34739 FOXA1 antibody [FOXA1/1518].
Blue : Primary antibody
Red : Isotype control
|
REFERENCE
There are currently no references for FOXA1 antibody [FOXA1/1518] (GTX34739). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for FOXA1 antibody [FOXA1/1518] (GTX34739). Be the first to share your experience with this product.
![Anti-FOXA1 antibody [FOXA1/1518] used in Western Blot (WB). GTX34739](/upload/website/prouct_img/small/GTX34739/GTX34739_20200115_WB_149_20011518_763.jpg "Anti-FOXA1 antibody [FOXA1/1518] used in Western Blot (WB). GTX34739")
![Anti-FOXA1 antibody [FOXA1/1518] used in IHC (Paraffin sections) (IHC-P). GTX34739](/upload/website/prouct_img/small/GTX34739/GTX34739_20200115_IHC-P_1849_20011518_965.jpg "Anti-FOXA1 antibody [FOXA1/1518] used in IHC (Paraffin sections) (IHC-P). GTX34739")
![Anti-FOXA1 antibody [FOXA1/1518] used in IHC (Paraffin sections) (IHC-P). GTX34739](/upload/website/prouct_img/small/GTX34739/GTX34739_20200115_IHC-P_1028_20011518_115.jpg "Anti-FOXA1 antibody [FOXA1/1518] used in IHC (Paraffin sections) (IHC-P). GTX34739")
![Anti-FOXA1 antibody [FOXA1/1518] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34739](/upload/website/prouct_img/small/GTX34739/GTX34739_20200115_ICC-IF_1746_20011518_867.jpg "Anti-FOXA1 antibody [FOXA1/1518] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34739")
![Anti-FOXA1 antibody [FOXA1/1518] used in Flow cytometry (FACS). GTX34739](/upload/website/prouct_img/small/GTX34739/GTX34739_20200115_FACS_1731_20011518_660.jpg "Anti-FOXA1 antibody [FOXA1/1518] used in Flow cytometry (FACS). GTX34739")
![Anti-FOXA1 antibody [FOXA1/1518] used in Western Blot (WB). GTX34739](/upload/website/prouct_img/normal/GTX34739/GTX34739_20200115_WB_149_20011518_763.jpg "Anti-FOXA1 antibody [FOXA1/1518] used in Western Blot (WB). GTX34739")
![Anti-FOXA1 antibody [FOXA1/1518] used in IHC (Paraffin sections) (IHC-P). GTX34739](/upload/website/prouct_img/normal/GTX34739/GTX34739_20200115_IHC-P_1849_20011518_965.jpg "Anti-FOXA1 antibody [FOXA1/1518] used in IHC (Paraffin sections) (IHC-P). GTX34739")
![Anti-FOXA1 antibody [FOXA1/1518] used in IHC (Paraffin sections) (IHC-P). GTX34739](/upload/website/prouct_img/normal/GTX34739/GTX34739_20200115_IHC-P_1028_20011518_115.jpg "Anti-FOXA1 antibody [FOXA1/1518] used in IHC (Paraffin sections) (IHC-P). GTX34739")
![Anti-FOXA1 antibody [FOXA1/1518] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34739](/upload/website/prouct_img/normal/GTX34739/GTX34739_20200115_ICC-IF_1746_20011518_867.jpg "Anti-FOXA1 antibody [FOXA1/1518] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34739")
![Anti-FOXA1 antibody [FOXA1/1518] used in Flow cytometry (FACS). GTX34739](/upload/website/prouct_img/normal/GTX34739/GTX34739_20200115_FACS_1731_20011518_660.jpg "Anti-FOXA1 antibody [FOXA1/1518] used in Flow cytometry (FACS). GTX34739")