Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXO1 antibody (GTX135251) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

FOXO1 antibody detects FOXO1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
FOXO1 stained by FOXO1 antibody (GTX135251) diluted at 1:5000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min