FSTL1 antibody [HL3523] detects FSTL1 protein by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FSTL1 stained by FSTL1 antibody [HL3523] (GTX641420) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

FSTL1 antibody [HL3523] detects secreted FSTL1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tissues.
FSTL1 stained by FSTL1 antibody [HL3523] (GTX641420) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Corresponding RNA levels (nTPM) in the tissues are based on Human Protein Atlas program.

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FSTL1 antibody [HL3523] (GTX641420) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.