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Factor H antibody [JA52-13]

Recombinant antibody
Anti-Factor H antibody [JA52-13] used in Western Blot (WB). GTX01032
Anti-Factor H antibody [JA52-13] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01032
Anti-Factor H antibody [JA52-13] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01032

Cat No. GTX01032

Host

Rabbit

Clonality

Monoclonal

Clone Name

JA52-13

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($369),
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Observed MW

150 kDa. The observed M.W. is based on the following publication. PMID: 10085008

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

complement factor H,AHUS1,AMBP1,ARMD4,ARMS1,CFH,CFHL3,FH,FHL1,HF,HF1,HF2,HUS,Factor H,Complement factor H

Cellular Localization

Secreted

Background

This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-Factor H antibody [JA52-13] used in Western Blot (WB). GTX01032

GTX01032 WB Image

Human plasma (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Factor H antibody [JA52-13] (GTX01032) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 10085008

Anti-Factor H antibody [JA52-13] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01032

GTX01032 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX01032 Factor H antibody [JA52-13].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-Factor H antibody [JA52-13] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01032

GTX01032 ICC/IF Image

ICC/IF analysis of PFA-fixed HepG2 cells using GTX01032 Factor H antibody [JA52-13].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

REFERENCE

There are currently no references for Factor H antibody [JA52-13] (GTX01032). Be the first to share your publications with this product.

REVIEW

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