Factor V antibody [HL2421] detects secreted Factor V protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
Factor V stained by Factor V antibody [HL2421] (GTX638643) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Human plasma (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Factor V antibody [HL2421] (GTX638643) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat plasma (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Factor V antibody [HL2421] (GTX638643) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.