Fascin 1 antibody [SPM133]

Cat. No. GTX34719

Host	Mouse
Clonality	Monoclonal
Clone Name	SPM133
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human
Package	100 μg ($529)

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Datasheet
Document

View product citations for antibody GTX34719 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2μg/ml
ICC/IF	1-2μg/ml
IHC-P	1-2μg/ml for 30 minutes at RT
FACS	1-2μg/10⁶ cells
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length recombinant human FSCN1 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FAN1 , FSCN1 , HSN , SNL , fascin actinbundling protein 1 , p55 , FASCIN 1 , fascin actin-bundling protein 1

Cellular Localization

Cytoplasm,Cytosol,Cytoskeleton,Cell projection,Filopodium,Invadopodium,Microvillus,Cell junction

Background

This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011]

Database

Gene ID: 6624 FSCN1
UniProt: Q16658 FSCN1

Research Area

DATA IMAGES

GTX34719 IHC-P Image

IHC-P analysis of human Hodgkin's lymph tissue using GTX34719 Fascin 1 antibody [SPM133].

GTX34719 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX34719 Fascin 1 antibody [SPM133].
Green : Primary antibody
Red : Phalloidin
Blue : nuclear counterstain

GTX34719 WB Image

WB analysis of HeLa cell lysate using GTX34719 Fascin 1 antibody [SPM133].

GTX34719 FACS Image

FACS analysis of PFA-fixed K562 cells using GTX34719 Fascin 1 antibody [SPM133].
Blue : Primary antibody
Red : Isotype control

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty