Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ferritin Heavy Chain protein stained by Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Human tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Ferritin Heavy Chain stained by Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Ferritin Heavy Chain stained by Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min