Sandwich ELISA analysis of recombinant human ferritin light polypeptide (FTL) standards ranging from 1.6-1000ng/ml using GTX15742 Ferritin antibody [521] (detection antibody) at 1 μg/mL and Ferritin duck polyclonal antibody (capture antibody).

Sandwich ELISA analysis of recombinant human ferritin light polypeptide (FTL) standards ranging from 1.6-1000ng/ml (A) or 100ul of diluted normal human serum or diluted normal human plasma (B) using GTX15742 Ferritin antibody [521] (capture antibody) at 1 μg/mL and biotin-conjugated ferritin rabbit polyclonal antibody (detection antibody) at 1 μg/mL.

ELISA analysis of human Ferritin or BSA diluted in carbonate/bicarbonate buffer (1 μg/ml, 100μl/well) using GTX15742 Ferritin antibody [521] at 2 - 1000 μg/mL (serial diluted).

ICC/IF analysis of HepG2 cells using GTX15742 Ferritin antibody [521]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:200 for at least 1 hour at room temperature