APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
PC-12 , 293T , H1299 , Molt-4 , Neuro2A , A549 nuclear extract , Jurkat nuclear extract , SK-N-SH nuclear extract , IMR32 nuclear extract
Predict Reactivity
Bovine, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.36 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Fibrillarin. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2036928
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
fibrillarin , FIB , FLRN , Nop1 , RNU3IP1
Cellular Localization
Nucleus , nucleolus
Background
This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX113684 IHC-P Image
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Fibrillarin antibody (GTX113684) at 1:300 dilution.
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GTX113684 WB Image
Sample (30 μg of whole cell lysate)
A: zebrafish eye
10% SDS PAGE
GTX113684 diluted at 1:1000
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GTX113684 ICC/IF Image
Fibrillarin antibody detects Fibrillarin protein at nucleus, nucleolus and cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Fibrillarin stained by Fibrillarin antibody (GTX113684) diluted at 1:2000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX113684 IHC-P Image
Fibrillarin antibody detects Fibrillarin protein at nucleolus in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
Fibrillarin antibody (GTX113684) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113684 WB Image
Fibrillarin antibody detects Fibrillarin protein by western blot analysis.
whole cell lysate (30 μg) and nuclear extract (30 μg) from IMR32 cells
10 % SDS-PAGE
Fibrillarin antibody (GTX113684) dilution: 1:500
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GTX113684 WB Image
Fibrillarin antibody detects Fibrillarin protein by western blot analysis.
whole cell lysate (30 μg) and nuclear extract(30 μg) from A549 cells
10 % SDS-PAGE
Fibrillarin antibody (GTX113684) dilution: 1:500
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GTX113684 WB Image
Fibrillarin antibody detects Fibrillarin protein by western blot analysis.
whole cell lysate (30 μg) and nuclear extract (30 μg) from Jurkat cells
10 % SDS-PAGE
Fibrillarin antibody (GTX113684) dilution: 1:500
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GTX113684 WB Image
Fibrillarin antibody detects Fibrillarin protein by western blot analysis.
whole cell lysate (30 μg) and nuclear extract (30 μg) from SK-N-SH cells
10 % SDS-PAGE
Fibrillarin antibody (GTX113684) dilution: 1:500
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GTX113684 IHC-P Image
Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using Fibrillarin (GTX113684) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113684 WB Image
Fibrillarin antibody detects FBL protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
12 % SDS-PAGE
Fibrillarin antibody (GTX113684) dilution: 1:500
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GTX113684 WB Image
Sample (30 ug of whole cell lysate)
A: 293T
B: H1299
C: Molt-4 (GTX27912)
12% SDS PAGE
GTX113684 diluted at 1:1000
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GTX113684 WB Image
Fibrillarin antibody detects FBL protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
12 % SDS-PAGE
Fibrillarin antibody (GTX113684) dilution: 1:1000
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REFERENCE
There are currently no references for Fibrillarin antibody (GTX113684). Be the first to share your publications with this product.
REVIEW
Fibrillarin antibody
Cat. No. GTX113684
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Rating
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( Average 4 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 05-Oct-2019
Application Tested :
WB
Sample Species :
Hu
Sample :
Huh7 cells
Amount used :
5 μg
Blocking :
2.5% non-fat milk, 25°C, 0.5Hr
Primary Antibody :
1:2000, 25°C, 4Hr
