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Fibrillarin antibody

Anti-Fibrillarin antibody used in Western Blot (WB). GTX113684
Anti-Fibrillarin antibody used in Western Blot (WB). GTX113684
Anti-Fibrillarin antibody used in Western Blot (WB). GTX113684
Anti-Fibrillarin antibody used in IHC (Paraffin sections) (IHC-P). GTX113684
Anti-Fibrillarin antibody used in IHC (Paraffin sections) (IHC-P). GTX113684
Anti-Fibrillarin antibody used in Western Blot (WB). GTX113684
Anti-Fibrillarin antibody used in Western Blot (WB). GTX113684
Anti-Fibrillarin antibody used in Western Blot (WB). GTX113684
Anti-Fibrillarin antibody used in Western Blot (WB). GTX113684
Anti-Fibrillarin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113684

Cat No. GTX113684

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T , H1299 , Molt-4 , Neuro2A , PC-12 , A549 , A549 nuclear extract , Jurkat , Jurkat nuclear extract , SK-N-SH , SK-N-SH nuclear extract , IMR32 , IMR32 nuclear extract

Predict Reactivity

Zebrafish, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fibrillarin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036928

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fibrillarin , FIB , FLRN , Nop1 , RNU3IP1

Cellular Localization

Nucleus , nucleolus

Background

This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Fibrillarin antibody used in Western Blot (WB). GTX113684

GTX113684 WB Image

Fibrillarin antibody detects Fibrillarin protein by western blot analysis.
whole cell lysate (30 μg) and nuclear extract(30 μg) from A549 cells
10 % SDS-PAGE
Fibrillarin antibody (GTX113684) dilution: 1:500

Anti-Fibrillarin antibody used in Western Blot (WB). GTX113684

GTX113684 WB Image

Fibrillarin antibody detects Fibrillarin protein by western blot analysis.
whole cell lysate (30 μg) and nuclear extract (30 μg) from SK-N-SH cells
10 % SDS-PAGE
Fibrillarin antibody (GTX113684) dilution: 1:500

Anti-Fibrillarin antibody used in Western Blot (WB). GTX113684

GTX113684 WB Image

Fibrillarin antibody detects Fibrillarin protein by western blot analysis.
whole cell lysate (30 μg) and nuclear extract (30 μg) from IMR32 cells
10 % SDS-PAGE
Fibrillarin antibody (GTX113684) dilution: 1:500

Anti-Fibrillarin antibody used in IHC (Paraffin sections) (IHC-P). GTX113684

GTX113684 IHC-P Image

Fibrillarin antibody detects Fibrillarin protein at nucleolus in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
Fibrillarin antibody (GTX113684) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Fibrillarin antibody used in IHC (Paraffin sections) (IHC-P). GTX113684

GTX113684 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using Fibrillarin (GTX113684) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Fibrillarin antibody used in Western Blot (WB). GTX113684

GTX113684 WB Image

Fibrillarin antibody detects FBL protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
12 % SDS-PAGE
Fibrillarin antibody (GTX113684) dilution: 1:1000

Anti-Fibrillarin antibody used in Western Blot (WB). GTX113684

GTX113684 WB Image

Fibrillarin antibody detects Fibrillarin protein by western blot analysis.
whole cell lysate (30 μg) and nuclear extract (30 μg) from Jurkat cells
10 % SDS-PAGE
Fibrillarin antibody (GTX113684) dilution: 1:500

Anti-Fibrillarin antibody used in Western Blot (WB). GTX113684

GTX113684 WB Image

Fibrillarin antibody detects FBL protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
12 % SDS-PAGE
Fibrillarin antibody (GTX113684) dilution: 1:500

Anti-Fibrillarin antibody used in Western Blot (WB). GTX113684

GTX113684 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: H1299
C: Molt-4 (GTX27912)
12% SDS PAGE
GTX113684 diluted at 1:1000

Anti-Fibrillarin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113684

GTX113684 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fibrillarin (GTX113684) antibody at 1:200 dilution.

REFERENCE

There are currently no references for Fibrillarin antibody (GTX113684). Be the first to share your publications with this product.

REVIEW

Fibrillarin antibody Cat No. GTX113684

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 05-Oct-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 5 μg
Blocking : 2.5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 25°C, 4Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169