Fibrinogen gamma antibody

Name: Fibrinogen gamma antibody
Brand: GeneTex
SKU: GTX108640
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Fibrinogen gamma antibody used in Immunoprecipitation (IP). GTX108640

Anti-Fibrinogen gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108640

Anti-Fibrinogen gamma antibody used in Western Blot (WB). GTX108640

Anti-Fibrinogen gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX108640

Cat. No. GTX108640

Reference 7

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat, Bovine
Package	100 μl ($399), 25 μl ($169)

See all Fibrinogen gamma products

View product citations for antibody GTX108640 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

rat plasma

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fibrinogen gamma. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950280

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fibrinogen gamma chain

Cellular Localization

Secreted

Background

The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Database

Gene ID: 2266 FGG
UniProt: P02679 FGG

Research Area

DATA IMAGES

GTX108640 IP Image

Immunoprecipitation of Fibrinogen gamma protein from HepG2 whole cell extracts using 5 μg of Fibrinogen gamma antibody (GTX108640).
Western blot analysis was performed using Fibrinogen gamma antibody (GTX108640).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX108640 ICC/IF Image

Fibrinogen gamma antibody detects Fibrinogen gamma protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Fibrinogen gamma protein stained by Fibrinogen gamma antibody (GTX108640) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

GTX108640 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody (GTX108640) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108640 WB Image

Mouse plasma and rat plasma (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody (GTX108640) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108640 WB Image

Human plasma and HepG2 conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody (GTX108640) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108640 IHC-P Image

Fibrinogen gamma antibody detects FGG protein at cytosol on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
Fibrinogen gamma antibody (GTX108640) dilution: 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108640 ICC/IF Image

Fibrinogen gamma antibody detects Fibrinogen gamma protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Fibrinogen gamma stained by Fibrinogen gamma antibody (GTX108640) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

REFERENCE

Application Reference

Lai H et al. Cancers (Basel) 2022; 14 (15) Protein Panel of Serum-Derived Small Extracellular Vesicles for the Screening and Diagnosis of Epithelial Ovarian Cancer.

Application : WB

Reactivity : Human

Revollo L et al. Blood 2022; 139 (1) : 104-117 The secreted tyrosine kinase VLK is essential for normal platelet activation and thrombus formation.

Matsuno Y et al. Biochem Biophys Res Commun 2022; 594 : 22-30 Characterization of lncRNA functioning in ovine conceptuses and endometria during the peri-implantation period.

Application : WB

Revollo L. et al. bioRxiv 2021; (Epub) The secreted tyrosine kinase VLK is essential for normal platelet activation and thrombus formation.

Application : WB

Reactivity : Human

Seto S et al. Front Microbiol 2019; 10 : 3081 Proteomic Profiling Reveals the Architecture of Granulomatous Lesions Caused by Tuberculosis and Mycobacterium avium Complex Lung Disease.

Application : IHC-P

Reactivity : Human

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