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Fibrinogen gamma antibody

Anti-Fibrinogen gamma antibody used in Immunoprecipitation (IP). GTX108640
Anti-Fibrinogen gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108640
Anti-Fibrinogen gamma antibody used in Western Blot (WB). GTX108640
Anti-Fibrinogen gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX108640
Anti-Fibrinogen gamma antibody used in Western Blot (WB). GTX108640
Anti-Fibrinogen gamma antibody used in Western Blot (WB). GTX108640
Anti-Fibrinogen gamma antibody used in Western Blot (WB). GTX108640

Cat No. GTX108640

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat, Bovine
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

HepG2 , human plasma , HepG2 sup. , mouse plasma , rat plasma

Predict Reactivity

Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fibrinogen gamma. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

fibrinogen gamma chain

Cellular Localization

Secreted

Background

The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Fibrinogen gamma antibody used in Immunoprecipitation (IP). GTX108640

GTX108640 IP Image

Immunoprecipitation of Fibrinogen gamma protein from HepG2 whole cell extracts using 5 μg of Fibrinogen gamma antibody (GTX108640).
Western blot analysis was performed using Fibrinogen gamma antibody (GTX108640).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Fibrinogen gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108640

GTX108640 ICC/IF Image

Fibrinogen gamma antibody detects Fibrinogen gamma protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Fibrinogen gamma protein stained by Fibrinogen gamma antibody (GTX108640) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

Anti-Fibrinogen gamma antibody used in Western Blot (WB). GTX108640

GTX108640 WB Image

Fibrinogen gamma antibody detects Fibrinogen gamma protein by western blot analysis. Human plasma and HepG2 conditioned medium were separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody (GTX108640) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fibrinogen gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX108640

GTX108640 IHC-P Image

Fibrinogen gamma antibody detects FGG protein at cytosol on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
Fibrinogen gamma antibody (GTX108640) dilution: 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Fibrinogen gamma antibody used in Western Blot (WB). GTX108640

GTX108640 WB Image

Fibrinogen gamma antibody detects FGG protein by western blot analysis.
A. 30 µg HepG2 whole cell lysate/extract
10% SDS-PAGE
Fibrinogen gamma antibody (GTX108640) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fibrinogen gamma antibody used in Western Blot (WB). GTX108640

GTX108640 WB Image

Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody (GTX108640) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fibrinogen gamma antibody used in Western Blot (WB). GTX108640

GTX108640 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody (GTX108640) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Fibrinogen gamma antibody Cat No. GTX108640

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 15-May-2018
Score :
Application Tested : WB
Sample Species : Bov
Blocking : 2% skim milk, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 18Hr
SDS
Glycerol.pdf
Thimerosal.pdf