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Fibronectin antibody [568]

Anti-Fibronectin antibody [568] used in IHC (Paraffin sections) (IHC-P). GTX34730

Cat No. GTX34730

Host

Mouse

Clonality

Monoclonal

Clone Name

568

Isotype

IgG1

Application

ICC/IF, IHC-P, FACS

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

263 kDa. ( Note )

Specificity/Sensitivity

Epitope of this MAb is located in the 8th type III repeat in the cell-binding region of fibronectin.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

High molecular weight proteins secreted by cultivated human fibroblasts

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CIG , EDB , FINC , FN , FN1 , FNZ , GFND , GFND2 , LETS , MSF , fibronectin 1 , Fibronectin

Cellular Localization

Secreted,Extracellular space,Extracellular matrix

Background

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-Fibronectin antibody [568] used in IHC (Paraffin sections) (IHC-P). GTX34730

GTX34730 IHC-P Image

IHC-P analysis of human renal cell carcinoma tissue using GTX34730 Fibronectin antibody [568].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf