APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 0.5-1ug/ml |
| 1-2ug/ml for 30 minutes at RT |
|
Note :
IHC-P
Boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
|
Not tested in other applications.
Calculated MW
Specificity/Sensitivity
Epitope of this MAb is located in the 8th type III repeat in the cell-binding region of fibronectin. Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb is excellent for staining of formalin-fixed, paraffin-embedded tissues.
PROPERTIES
Form
Liquid
Buffer
10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml
Antigen Species
Human
Immunogen
High molecular weight proteins secreted by cultivated human fibroblasts
Purification
Ab purified from Bioreactor Concentrate by Protein A/G
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Fibronectin 1 , Cig , Ed-B , Finc , Fn , Fnz , Gfnd , Gfnd2 , Lets , Msf , Smdcf , Fn1
Background
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Fibronectin antibody [568] used in IHC (Paraffin sections) (IHC-P). GTX34730](/upload/website/prouct_img/middle/GTX34730/GTX34730_20170712_IHC-P_18121410_697.jpg "Anti-Fibronectin antibody [568] used in IHC (Paraffin sections) (IHC-P). GTX34730")
|
GTX34730 IHC-P Image
Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Fibronectin Monoclonal Antibody (568).
|
REFERENCE
There are currently no references for Fibronectin antibody [568] (GTX34730). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Fibronectin antibody [568] (GTX34730). Be the first to share your experience with this product.
![Anti-Fibronectin antibody [568] used in IHC (Paraffin sections) (IHC-P). GTX34730](/upload/website/prouct_img/small/GTX34730/GTX34730_20170712_IHC-P_18121410_697.jpg "Anti-Fibronectin antibody [568] used in IHC (Paraffin sections) (IHC-P). GTX34730")
![Anti-Fibronectin antibody [568] used in IHC (Paraffin sections) (IHC-P). GTX34730](/upload/website/prouct_img/normal/GTX34730/GTX34730_20170712_IHC-P_18121410_697.jpg "Anti-Fibronectin antibody [568] used in IHC (Paraffin sections) (IHC-P). GTX34730")
