GeneTex
United States (US)

Fibronectin antibody [SPM539]

GTX34726_20170712_IHC-P_18121410_137.jpg

Cat No. GTX34726

Host Mouse
Clonality Monoclonal
Clone Name SPM539
Isotype IgG1
Application IHC-P
Reactivity Human
APPLICATION

Application Note

IHC-P:0.5-1ug/ml for 30 minutes at RT.

Calculated MW

263 kDa. ( Note )

Specificity/Sensitivity

Fibronectin is a dimeric glycoprotein of 440kDa, which is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing. This MAb is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin. It blocks the fibronectin-medicated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Human fibronectin purified from serum by affinity chromatography on gelatin-sepharose

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fibronectin 1,Cig,Ed-B,Finc,Fn,Fnz,Gfnd,Gfnd2,Lets,Msf,Smdcf,Fn1

Background

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX34726_20170712_IHC-P_18121410_137.jpg

GTX34726 IHC-P Image

Formalin-fixed, paraffin-embedded human Pancreatic Adenocarcinoma stained with Fibronectin Monoclonal Antibody (SPM539).

Package List Price ($)
$ 329