APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Product Note
This MAb is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin. It blocks the fibronectin-medicated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human fibronectin Purified from serum by affinity chromatography on gelatin-sepharose
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CIG , EDB , FINC , FN , FN1 , FNZ , GFND , GFND2 , LETS , MSF , fibronectin 1 , Fibronectin
Cellular Localization
Secreted,Extracellular space,Extracellular matrix
Background
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]
Database
Research Area
DATA IMAGES
![Anti-Fibronectin antibody [SPM539] used in IHC (Paraffin sections) (IHC-P). GTX34726](/templates/default/js/lightbox/images/loading.gif "Anti-Fibronectin antibody [SPM539] used in IHC (Paraffin sections) (IHC-P). GTX34726")
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GTX34726 IHC-P Image
IHC-P analysis of human pancreatic adenocarcinoma tissue using GTX34726 Fibronectin antibody [SPM539].
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REFERENCE
There are currently no references for Fibronectin antibody [SPM539] (GTX34726). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Fibronectin antibody [SPM539] (GTX34726). Be the first to share your experience with this product.
![Anti-Fibronectin antibody [SPM539] used in IHC (Paraffin sections) (IHC-P). GTX34726](/upload/website/prouct_img/small/GTX34726/GTX34726_20200115_IHC-P_910_w_23060801_579.webp "Anti-Fibronectin antibody [SPM539] used in IHC (Paraffin sections) (IHC-P). GTX34726")
![Anti-Fibronectin antibody [SPM539] used in IHC (Paraffin sections) (IHC-P). GTX34726](/upload/website/prouct_img/normal/GTX34726/GTX34726_20200115_IHC-P_910_w_23060801_579.webp "Anti-Fibronectin antibody [SPM539] used in IHC (Paraffin sections) (IHC-P). GTX34726")