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G9a antibody

KO/KD
Anti-G9a antibody used in Western Blot (WB). GTX128164
Anti-G9a antibody used in IHC (Paraffin sections) (IHC-P). GTX128164
Anti-G9a antibody used in Western Blot (WB). GTX128164
Anti-G9a antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128164
Anti-G9a antibody used in IHC (Paraffin sections) (IHC-P). GTX128164
Anti-G9a antibody used in IHC (Paraffin sections) (IHC-P). GTX128164
Anti-G9a antibody used in Immunoprecipitation (IP). GTX128164

Cat. No. GTX128164

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all G9a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:1000
Not tested in other applications.

Calculated MW

132 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human G9a. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885719

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

euchromatic histone lysine methyltransferase 2 , BAT8 , C6orf30 , G9A , GAT8 , KMT1C , NG36

Cellular Localization

Nucleus

Background

A cluster of genes, BAT1-BAT5, has been localized in the vicinity of the genes for TNF alpha and TNF beta. This gene is found near this cluster; it was mapped near the gene for C2 within a 120-kb region that included a HSP70 gene pair. These genes are all within the human major histocompatibility complex class III region. This gene was thought to be two different genes, NG36 and G9a, adjacent to each other but a recent publication shows that there is only a single gene. The protein encoded by this gene is thought to be involved in intracellular protein-protein interaction. There are three alternatively spliced transcript variants of this gene but only two are fully described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-G9a antibody used in Western Blot (WB). GTX128164

GTX128164 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX128164) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-G9a antibody used in IHC (Paraffin sections) (IHC-P). GTX128164

GTX128164 IHC-P Image

G9a antibody detects G9a protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
G9a stained by G9a antibody (GTX128164) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-G9a antibody used in Western Blot (WB). GTX128164

GTX128164 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX128164) diluted at 1:1000.

Anti-G9a antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128164

GTX128164 ICC/IF Image

G9a antibody detects EHMT2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 5 min.
Green: EHMT2 protein stained by G9a antibody (GTX128164) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-G9a antibody used in IHC (Paraffin sections) (IHC-P). GTX128164

GTX128164 IHC-P Image

G9a antibody detects EHMT2 protein at on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
G9a antibody (GTX128164) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-G9a antibody used in IHC (Paraffin sections) (IHC-P). GTX128164

GTX128164 IHC-P Image

G9a antibody detects G9a protein at nucleus in human rectal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human rectal carcinoma.
G9a antibody (GTX128164) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-G9a antibody used in Immunoprecipitation (IP). GTX128164

GTX128164 IP Image

Immunoprecipitation of G9a protein from HeLa whole cell extracts using 5 μg of G9a antibody (GTX128164).
Western blot analysis was performed using G9a antibody (GTX128164) diluted at 1:1000.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

Application Reference

Yang IP et al. Ther Adv Med Oncol 2019; 11 : 1.76E+15
Application : WB
Reactivity : Human
Liu G et al. Mol Med Rep 2015; 12 (3) : 3297-308 Comparison of the neuropsychological mechanisms of 2,6-diisopropylphenol and N-methyl-D-aspartate receptor antagonist against electroconvulsive therapy-induced learning and memory impairment in depressed rats.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169