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G9a antibody

Anti-G9a antibody used in Western Blot (WB). GTX128164
Anti-G9a antibody used in Immunoprecipitation (IP). GTX128164
Anti-G9a antibody used in IHC (Paraffin sections) (IHC-P). GTX128164
Anti-G9a antibody used in IHC (Paraffin sections) (IHC-P). GTX128164
Anti-G9a antibody used in Western Blot (WB). GTX128164
Anti-G9a antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128164

Cat No. GTX128164

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:1000
Not tested in other applications.

Calculated MW

132 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human G9a. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

euchromatic histone lysine methyltransferase 2,BAT8,C6orf30,G9A,GAT8,KMT1C,NG36

Cellular Localization

Nucleus

Background

A cluster of genes, BAT1-BAT5, has been localized in the vicinity of the genes for TNF alpha and TNF beta. This gene is found near this cluster; it was mapped near the gene for C2 within a 120-kb region that included a HSP70 gene pair. These genes are all within the human major histocompatibility complex class III region. This gene was thought to be two different genes, NG36 and G9a, adjacent to each other but a recent publication shows that there is only a single gene. The protein encoded by this gene is thought to be involved in intracellular protein-protein interaction. There are three alternatively spliced transcript variants of this gene but only two are fully described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-G9a antibody used in Western Blot (WB). GTX128164

GTX128164 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX128164) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-G9a antibody used in Immunoprecipitation (IP). GTX128164

GTX128164 IP Image

Immunoprecipitation of G9a protein from HeLa whole cell extracts using 5 μg of G9a antibody (GTX128164).
Western blot analysis was performed using G9a antibody (GTX128164).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-G9a antibody used in IHC (Paraffin sections) (IHC-P). GTX128164

GTX128164 IHC-P Image

G9a antibody detects EHMT2 protein at on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
G9a antibody (GTX128164) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-G9a antibody used in IHC (Paraffin sections) (IHC-P). GTX128164

GTX128164 IHC-P Image

G9a antibody detects G9a protein at nucleus in human rectal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human rectal carcinoma.
G9a antibody (GTX128164) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-G9a antibody used in Western Blot (WB). GTX128164

GTX128164 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX128164) diluted at 1:1000.

Anti-G9a antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128164

GTX128164 ICC/IF Image

G9a antibody detects EHMT2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 5 min.
Green: EHMT2 protein stained by G9a antibody (GTX128164) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169