Boiled and unboiled mouse tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GABA A Receptor alpha 1 antibody [GT154] (GTX642581) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Boiled and unboiled rat tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GABA A Receptor alpha 1 antibody [GT154] (GTX642581) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.