Boiled and unboiled rat tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GABA Transporter 1 / GAT1 antibody [HL3475] (GTX641360) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GABA Transporter 1 / GAT1 antibody [HL3475] detects GABA Transporter 1 / GAT1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse eye.
Green: GABA Transporter 1 / GAT1 stained by GABA Transporter 1 / GAT1 antibody [HL3475] (GTX641360) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GABA Transporter 1 / GAT1 antibody [HL3475] detects GABA Transporter 1 / GAT1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: GABA Transporter 1 / GAT1 stained by GABA Transporter 1 / GAT1 antibody [HL3475] (GTX641360) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GABA Transporter 1 / GAT1 antibody [HL3475] detects GABA Transporter 1 / GAT1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat eye.
Green: GABA Transporter 1 / GAT1 stained by GABA Transporter 1 / GAT1 antibody [HL3475] (GTX641360) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GABA Transporter 1 / GAT1 antibody [HL3475] detects GABA Transporter 1 / GAT1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tissues.
GABA Transporter 1 / GAT1 stained by GABA Transporter 1 / GAT1 antibody [HL3475] (GTX641360) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Corresponding RNA levels (RPKM) in the tissues are based on NCBI database.

Unboiled human tissue extract was separated by 7.5% SDS-PAGE, and the membrane was blotted with GABA Transporter 1 / GAT1 antibody [HL3475] (GTX641360) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Unboiled various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GABA Transporter 1 / GAT1 antibody [HL3475] (GTX641360) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.