APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
rat cerebellum
Predict Reactivity
Human, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.53 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Recombinant protein encompassing a sequence within the center region of mouse GABA Transporter 1 / GAT1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886844
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
solute carrier family 6 (neurotransmitter transporter, GABA), member 1 , A730043E01 , GABATHG , GABATR , GAT-1 , Gabt , Gabt1 , Gat1 , XT-1 , Xtrp1
Cellular Localization
Cell membrane; Multi-pass membrane protein , Membrane; Multi-pass membrane protein
Background
Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
Database
Research Area
DATA IMAGES
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GTX133150 IHC-P Image
GABA Transporter 1 / GAT1 antibody detects GABA Transporter 1 / GAT1 protein at cell membrane and cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GABA Transporter 1 / GAT1 antibody (GTX133150) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133150 IHC-P Image
GABA Transporter 1 / GAT1 antibody detects GABA Transporter 1 / GAT1 protein at cell membrane and cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GABA Transporter 1 / GAT1 antibody (GTX133150) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133150 WB Image
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GABA Transporter 1 / GAT1 antibody (GTX133150) diluted at 1:500.
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GTX133150 IHC-Fr Image
GABA Transporter 1 / GAT1 antibody detects GABA Transporter 1 / GAT1 protein by immunohistochemical analysis.
Samples: Frozen Sectioned adult mouse hippocampus.
Green: GABA Transporter 1 / GAT1 protein stained by GABA Transporter 1 / GAT1 antibody (GTX133150) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
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GTX133150 ICC/IF Image
GABA Transporter 1 / GAT1 antibody detects GABA Transporter 1 / GAT1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GABA Transporter 1 / GAT1 stained by GABA Transporter 1 / GAT1 antibody (GTX133150) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
REVIEW
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