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GAD65 + GAD67 antibody [HL1181]

Recombinant antibody Overexpression
Anti-GAD65 + GAD67 antibody [HL1181] used in IHC (Paraffin sections) (IHC-P). GTX636487
Anti-GAD65 + GAD67 antibody [HL1181] used in Western Blot (WB). GTX636487
Anti-GAD65 + GAD67 antibody [HL1181] used in Western Blot (WB). GTX636487
Anti-GAD65 + GAD67 antibody [HL1181] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636487
Anti-GAD65 + GAD67 antibody [HL1181] used in Western Blot (WB). GTX636487
Anti-GAD65 + GAD67 antibody [HL1181] used in Western Blot (WB). GTX636487
Anti-GAD65 + GAD67 antibody [HL1181] used in Western Blot (WB). GTX636487
Anti-GAD65 + GAD67 antibody [HL1181] used in IHC (Paraffin sections) (IHC-P). GTX636487
Anti-GAD65 + GAD67 antibody [HL1181] used in IHC (Paraffin sections) (IHC-P). GTX636487

Cat. No. GTX636487

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1181

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all GAD65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GAD65. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909993

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glutamate decarboxylase 2 , GAD65

Cellular Localization

Cytoplasm, cytosol,Cytoplasmic vesicle,Golgi apparatus membrane

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-GAD65 + GAD67 antibody [HL1181] used in IHC (Paraffin sections) (IHC-P). GTX636487

GTX636487 IHC-P Image

GAD65 + GAD67 antibody [HL1181] detects GAD65 + GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: GAD65 + GAD67 stained by GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAD65 + GAD67 antibody [HL1181] used in Western Blot (WB). GTX636487

GTX636487 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD65 + GAD67 antibody [HL1181] used in Western Blot (WB). GTX636487

GTX636487 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD65 + GAD67 antibody [HL1181] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636487

GTX636487 ICC/IF Image

GAD65 + GAD67 antibody [HL1181] detects GAD65 + GAD67 protein at cytosol and cytoskeleton by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAD65 + GAD67 stained by GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GAD65 + GAD67 antibody [HL1181] used in Western Blot (WB). GTX636487

GTX636487 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD65 + GAD67 antibody [HL1181] used in Western Blot (WB). GTX636487

GTX636487 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD65 + GAD67 antibody [HL1181] used in Western Blot (WB). GTX636487

GTX636487 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD65 + GAD67 antibody [HL1181] used in IHC (Paraffin sections) (IHC-P). GTX636487

GTX636487 IHC-P Image

GAD65 + GAD67 antibody [HL1181] detects GAD65 + GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog brain.
GAD65 + GAD67 stained by GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAD65 + GAD67 antibody [HL1181] used in IHC (Paraffin sections) (IHC-P). GTX636487

GTX636487 IHC-P Image

GAD65 + GAD67 antibody [HL1181] detects GAD65 + GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat cerebellum.
GAD65 + GAD67 stained by GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations