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GAD65 + GAD67 antibody [HL1181]

Anti-GAD65 + GAD67 antibody [HL1181] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636487
Anti-GAD65 + GAD67 antibody [HL1181] used in IHC (Paraffin sections) (IHC-P). GTX636487
Anti-GAD65 + GAD67 antibody [HL1181] used in IHC (Paraffin sections) (IHC-P). GTX636487
Anti-GAD65 + GAD67 antibody [HL1181] used in Western Blot (WB). GTX636487
Anti-GAD65 + GAD67 antibody [HL1181] used in IHC (Paraffin sections) (IHC-P). GTX636487
Anti-GAD65 + GAD67 antibody [HL1181] used in Western Blot (WB). GTX636487
Anti-GAD65 + GAD67 antibody [HL1181] used in Western Blot (WB). GTX636487
Anti-GAD65 + GAD67 antibody [HL1181] used in Western Blot (WB). GTX636487
Anti-GAD65 + GAD67 antibody [HL1181] used in Western Blot (WB). GTX636487

Cat. No. GTX636487

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1181

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Zebrafish, Cat, Dog
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GAD65. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909993

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate decarboxylase 2 , GAD65

Cellular Localization

Cytoplasm, cytosol,Cytoplasmic vesicle,Golgi apparatus membrane

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-GAD65 + GAD67 antibody [HL1181] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636487

GTX636487 ICC/IF Image

GAD65 + GAD67 antibody [HL1181] detects GAD65 + GAD67 protein at cytosol and cytoskeleton by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAD65 + GAD67 stained by GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GAD65 + GAD67 antibody [HL1181] used in IHC (Paraffin sections) (IHC-P). GTX636487

GTX636487 IHC-P Image

GAD65 + GAD67 antibody [HL1181] detects GAD65 + GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog brain.
GAD65 + GAD67 stained by GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAD65 + GAD67 antibody [HL1181] used in IHC (Paraffin sections) (IHC-P). GTX636487

GTX636487 IHC-P Image

GAD65 + GAD67 antibody [HL1181] detects GAD65 + GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat cerebellum.
GAD65 + GAD67 stained by GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAD65 + GAD67 antibody [HL1181] used in Western Blot (WB). GTX636487

GTX636487 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD65 + GAD67 antibody [HL1181] used in IHC (Paraffin sections) (IHC-P). GTX636487

GTX636487 IHC-P Image

GAD65 + GAD67 antibody [HL1181] detects GAD65 + GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: GAD65 + GAD67 stained by GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAD65 + GAD67 antibody [HL1181] used in Western Blot (WB). GTX636487

GTX636487 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD65 + GAD67 antibody [HL1181] used in Western Blot (WB). GTX636487

GTX636487 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD65 + GAD67 antibody [HL1181] used in Western Blot (WB). GTX636487

GTX636487 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD65 + GAD67 antibody [HL1181] used in Western Blot (WB). GTX636487

GTX636487 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations