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GAD65 antibody [HL1187]

Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493
Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493
Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493
Anti-GAD65 antibody [HL1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636493
Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493

Cat No. GTX636493

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1187

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($369),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Product Note

This antibody is specific for GAD65 protein, and it does not cross-react with GAD67 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GAD65. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909995

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glutamate decarboxylase 2 , GAD65

Cellular Localization

Cytoplasm, cytosol,Cytoplasmic vesicle,Golgi apparatus membrane

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493

GTX636493 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493

GTX636493 IHC-P Image

GAD65 antibody [HL1187] detects GAD65 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: GAD65 stained by GAD65 antibody [HL1187] (GTX636493) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493

GTX636493 WB Image

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD65 antibody [HL1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636493

GTX636493 ICC/IF Image

GAD65 antibody [HL1187] detects GAD65 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAD65 stained by GAD65 antibody [HL1187] (GTX636493) diluted at 1:500.
Red: Tau, a Tau marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493

GTX636493 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 369
$ 169
Custom Conjugations