GAD67 antibody [HL1096]

GAD67 antibody [HL1096] detects GAD67 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.
Green: GAD67 stained by GAD67 antibody [HL1096] (GTX636280) diluted at 1:200.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1096] (GTX636280) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1096] (GTX636280) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GAD67 antibody [HL1096] detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GAD67 stained by GAD67 antibody [HL1096] (GTX636280) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GAD67 antibody [HL1096] detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GAD67 stained by GAD67 antibody [HL1096] (GTX636280) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1096] (GTX636280) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Human tissue extract (10 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1096] (GTX636280) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GAD67 antibody [HL1096] detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: GAD67 stained by GAD67 antibody [HL1096] (GTX636280) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a nerual marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GAD67 antibody [HL1096] detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog cerebellum.
GAD67 stained by GAD67 antibody [HL1096] (GTX636280) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GAD67 antibody [HL1096] detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat cerebellum.
GAD67 stained by GAD67 antibody [HL1096] (GTX636280) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole Japanese medaka extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1096] (GTX636280) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.