GeneTex
United States (US)

GAD67 antibody

Overexpression
GTX101881_41396_20160921_IHC-Fr_19011506_992.jpg
GTX101881_39596_IHC_M_19011506_478.jpg
GTX101881_42529_20160818_WB_M_R_19011506_378.jpg
GTX101881_42557_20160830_IHC-P_R_19011506_411.jpg
GTX101881_42557_20160721_WB_19011506_942.jpg
GTX101881_41396_WB_B_19011506_651.jpg
GTX101881_42529_20161109_IFA_19011506_404.jpg
GTX101881_42529_20160630_WB_19011506_670.jpg
GTX101881_41396_20160910_IHC-Fr_19011506_460.jpg
GTX101881_42557_20160818_WB_M_R_19011506_559.jpg
GTX101881_42557_20180824_WB_19011506_287.jpg

Cat No. GTX101881

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

SK-N-SH , mouse brain , rat brain , human GAD1-transfected 293T

Predict Reactivity

Bovine, Cat, Dog, Chicken, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human GAD67. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glutamate Decarboxylase 1,Cpsq1,Gad,Scp,Gad1

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101881_41396_20160921_IHC-Fr_19011506_992.jpg

GTX101881 IHC-Fr Image

GAD67 antibody detects GAD67 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: GAD67 protein stained by GAD67 antibody (GTX101881) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101881_39596_IHC_M_19011506_478.jpg

GTX101881 IHC-P Image

GAD67 antibody detects GAD67 protein at on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
GAD67 antibody (GTX101881) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101881_42529_20160818_WB_M_R_19011506_378.jpg

GTX101881 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:1000.

GTX101881_42557_20160830_IHC-P_R_19011506_411.jpg

GTX101881 IHC-P Image

GAD67 antibody detects GAD67 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GAD67 antibody (GTX101881) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101881_42557_20160721_WB_19011506_942.jpg

GTX101881 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:500.

GTX101881_41396_WB_B_19011506_651.jpg

GTX101881 WB Image

Overexpression

GAD67 antibody detects GAD67 protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of human GAD1-transfected 293T cells
7.5% SDS-PAGE
GAD67 antibody (GTX101881) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101881_42529_20161109_IFA_19011506_404.jpg

GTX101881 ICC/IF Image

GAD67 antibody detects GAD67 protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAD67 protein stained by GAD67 antibody (GTX101881) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX101881_42529_20160630_WB_19011506_670.jpg

GTX101881 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:500.

GTX101881_41396_20160910_IHC-Fr_19011506_460.jpg

GTX101881 IHC-Fr Image

GAD67 antibody detects GAD67 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: GAD67 protein stained by GAD67 antibody (GTX101881) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101881_42557_20160818_WB_M_R_19011506_559.jpg

GTX101881 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:1000.

GTX101881_42557_20180824_WB_19011506_287.jpg

GTX101881 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 (GTX101881) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169