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GAD67 antibody

Overexpression
Anti-GAD67 antibody used in Western Blot (WB). GTX101881
Anti-GAD67 antibody used in Western Blot (WB). GTX101881
Anti-GAD67 antibody used in IHC (Paraffin sections) (IHC-P). GTX101881
Anti-GAD67 antibody used in Western Blot (WB). GTX101881
Anti-GAD67 antibody used in IHC (Paraffin sections) (IHC-P). GTX101881
Anti-GAD67 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101881
Anti-GAD67 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101881
Anti-GAD67 antibody used in IHC (Paraffin sections) (IHC-P). GTX101881
Anti-GAD67 antibody used in Western Blot (WB). GTX101881
Anti-GAD67 antibody used in IHC (Paraffin sections) (IHC-P). GTX101881
Anti-GAD67 antibody used in IHC (Paraffin sections) (IHC-P). GTX101881
Anti-GAD67 antibody used in Western Blot (WB). GTX101881
Anti-GAD67 antibody used in Immunohistochemistry (IHC). GTX101881
Anti-GAD67 antibody used in IHC (Whole mount) (IHC-Wm). GTX101881

Cat. No. GTX101881

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IHC, IHC (Free Floating)

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all GAD67 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC-Wm Assay dependent
IHC Assay dependent
IHC (Free Floating) Assay dependent
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

human GAD1-transfected 293T

Product Note

This antibody is specific for GAD67 protein, and it does not cross-react with GAD65 protein.

Predict Reactivity

Bovine, Cat, Dog, Chicken, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human GAD67. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240876

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate decarboxylase 1 , CPSQ1 , GAD , SCP

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GAD67 antibody used in Western Blot (WB). GTX101881

GTX101881 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD67 antibody used in Western Blot (WB). GTX101881

GTX101881 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD67 antibody used in IHC (Paraffin sections) (IHC-P). GTX101881

GTX101881 IHC-P Image

GAD67 antibody detects GAD67 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAD67 antibody used in Western Blot (WB). GTX101881

GTX101881 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD67 antibody used in IHC (Paraffin sections) (IHC-P). GTX101881

GTX101881 IHC-P Image

GAD67 antibody detects GAD67 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAD67 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101881

GTX101881 ICC/IF Image

Overexpression

GAD67 antibody detects GAD67 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GAD67 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101881

GTX101881 ICC/IF Image

GAD67 antibody detects GAD67 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GAD67 antibody used in IHC (Paraffin sections) (IHC-P). GTX101881

GTX101881 IHC-P Image

GAD67 antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAD67 antibody used in Western Blot (WB). GTX101881

GTX101881 WB Image

Overexpression

GAD67 antibody detects GAD67 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human GAD1-transfected 293T cells
7.5% SDS-PAGE
GAD67 antibody (GTX101881) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD67 antibody used in IHC (Paraffin sections) (IHC-P). GTX101881

GTX101881 IHC-P Image

GAD67 antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAD67 antibody used in IHC (Paraffin sections) (IHC-P). GTX101881

GTX101881 IHC-P Image

GAD67 antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAD67 antibody used in Western Blot (WB). GTX101881

GTX101881 WB Image

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD67 antibody used in Immunohistochemistry (IHC). GTX101881

GTX101881 IHC Image

GAD67 antibody detects GAD67 protein on myofiber membranes by immunohistochemical analysis.
Sample: Agarose-embedded zebrafish embryo
GAD67 antibody (GTX101881) dilution: 1:200.
Image provided with permission courtesy of Dr. T. Schilling at UC, Irvine.

Anti-GAD67 antibody used in IHC (Whole mount) (IHC-Wm). GTX101881

GTX101881 IHC-Wm Image

GAD67 antibody detects GAD67 protein on myofiber membranes by immunohistochemical analysis.
Sample: Whole-mount zebrafish embryo
GAD67 antibody (GTX101881) dilution: 1:200.

REFERENCE

Application Reference

Borkowska P et al. Biomedicines 2023; 11 (6) Effects of the Co-Overexpression of the BCL and BDNF Genes on the Gamma-Aminobutyric Acid-Ergic Differentiation of Wharton's-Jelly-Derived Mesenchymal Stem Cells.
Paulina Borkowska et al. Biomedicines 2023; 11 : 1751 Effects of the Co-Overexpression of the?BCL?and?BDNF?Genes on the Gamma-Aminobutyric Acid-Ergic Differentiation of Wharton’s-Jelly-Derived Mesenchymal Stem Cells
Application : ICC/IF
Tsai CW et al. Neurotherapeutics 2022; 19 (2) : 649-659 Transcranial Ultrasound Stimulation Reverses Behavior Changes and the Expression of Calcium-Binding Protein in a Rodent Model of Schizophrenia.
Ji MH et al. Brain Behav Immun 2020; 89 : 133-144 Neural network disturbance in the medial prefrontal cortex might contribute to cognitive impairments induced by neuroinflammation.
Application : IHC-Fr
Reactivity : Rat
Ma X et al. J Endocrinol 2020; 246 (3) : 223-236 ACE2 modulates glucose homeostasis through GABA signaling during metabolic stress.
Application : IHC-Fr
Reactivity : Rat
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REVIEW

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Application Reference
Borkowska P et al. Biomedicines 2023; 11 (6) Effects of the Co-Overexpression of the BCL and BDNF Genes on the Gamma-Aminobutyric Acid-Ergic Differentiation of Wharton's-Jelly-Derived Mesenchymal Stem Cells.
Paulina Borkowska et al. Biomedicines 2023; 11:1751 Effects of the Co-Overexpression of the?BCL?and?BDNF?Genes on the Gamma-Aminobutyric Acid-Ergic Differentiation of Wharton’s-Jelly-Derived Mesenchymal Stem Cells
Application : ICC/IF
Tsai CW et al. Neurotherapeutics 2022; 19 (2):649-659 Transcranial Ultrasound Stimulation Reverses Behavior Changes and the Expression of Calcium-Binding Protein in a Rodent Model of Schizophrenia.
Ji MH et al. Brain Behav Immun 2020; 89:133-144 Neural network disturbance in the medial prefrontal cortex might contribute to cognitive impairments induced by neuroinflammation.
Application : IHC-Fr
Reactivity : Rat
Ma X et al. J Endocrinol 2020; 246 (3):223-236 ACE2 modulates glucose homeostasis through GABA signaling during metabolic stress.
Application : IHC-Fr
Reactivity : Rat
Wang C et al. Neuroscience 2019; 404:268-281 Two Groups of eGFP-Expressing Neurons with Distinct Characteristics in the Neocortex of GIN Mice.
Application : IHC (Free Floating)
Reactivity : Mouse
Occelli F et al. Neuroscience 2019; 404:184-204 Age-related Changes in Auditory Cortex Without Detectable Peripheral Alterations: A Multi-level Study in Sprague-Dawley Rats.
Application : IHC-P
Reactivity : Rat
Zhou Z et al. Mol Neurobiol 2014; loss of phenotype of parvalbumin interneurons in rat prefrontal cortex is involved in antidepressant- and propsychotic-like behaviors following acute and repeated * administration.
Application : WB
Reactivity : Rat
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169