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GAD67 antibody

Overexpression
Anti-GAD67 antibody used in IHC (Frozen sections) (IHC-Fr). GTX101881
Anti-GAD67 antibody used in IHC (Paraffin sections) (IHC-P). GTX101881
Anti-GAD67 antibody used in IHC (Paraffin sections) (IHC-P). GTX101881
Anti-GAD67 antibody used in Western Blot (WB). GTX101881
Anti-GAD67 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101881
Anti-GAD67 antibody used in IHC (Frozen sections) (IHC-Fr). GTX101881
Anti-GAD67 antibody used in Western Blot (WB). GTX101881
Anti-GAD67 antibody used in Western Blot (WB). GTX101881

Cat No. GTX101881

Reference 3
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IHC
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
See all GAD67 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

SK-N-SH , mouse brain , rat brain , human GAD1-transfected 293T

Predict Reactivity

Bovine, Cat, Dog, Chicken, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human GAD67. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

glutamate decarboxylase 1 , CPSQ1 , GAD , SCP

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-GAD67 antibody used in IHC (Frozen sections) (IHC-Fr). GTX101881

GTX101881 IHC-Fr Image

GAD67 antibody detects GAD67 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: GAD67 protein stained by GAD67 antibody (GTX101881) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-GAD67 antibody used in IHC (Paraffin sections) (IHC-P). GTX101881

GTX101881 IHC-P Image

GAD67 antibody detects GAD67 protein at on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
GAD67 antibody (GTX101881) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Anti-GAD67 antibody used in IHC (Paraffin sections) (IHC-P). GTX101881

GTX101881 IHC-P Image

GAD67 antibody detects GAD67 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GAD67 antibody (GTX101881) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Anti-GAD67 antibody used in Western Blot (WB). GTX101881

GTX101881 WB Image

Overexpression

GAD67 antibody detects GAD67 protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of human GAD1-transfected 293T cells
7.5% SDS-PAGE
GAD67 antibody (GTX101881) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-GAD67 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101881

GTX101881 ICC/IF Image

GAD67 antibody detects GAD67 protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAD67 protein stained by GAD67 antibody (GTX101881) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-GAD67 antibody used in IHC (Frozen sections) (IHC-Fr). GTX101881

GTX101881 IHC-Fr Image

GAD67 antibody detects GAD67 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: GAD67 protein stained by GAD67 antibody (GTX101881) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-GAD67 antibody used in Western Blot (WB). GTX101881

GTX101881 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-GAD67 antibody used in Western Blot (WB). GTX101881

GTX101881 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
REFERENCE

Application Reference

Wang C et al. Neuroscience 2019; 404 : 268-281 Two Groups of eGFP-Expressing Neurons with Distinct Characteristics in the Neocortex of GIN Mice.
Application : IHC
Reactivity : Mouse
Occelli F et al. Neuroscience 2019; 404 : 184-204 Age-related Changes in Auditory Cortex Without Detectable Peripheral Alterations: A Multi-level Study in Sprague-Dawley Rats.
Application : IHC-P
Reactivity : Rat
Zhou Z et al. Mol Neurobiol 2014; loss of phenotype of parvalbumin interneurons in rat prefrontal cortex is involved in antidepressant- and propsychotic-like behaviors following acute and repeated * administration.
Application : WB
Reactivity : Rat
Submit a Reference
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169