APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
SK-N-SH , mouse brain , rat brain , human GAD1-transfected 293T
Product Note
This antibody is specific for GAD67 protein, and it does not cross-react with GAD65 protein.
Predict Reactivity
Bovine, Cat, Dog, Chicken, Pig, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS pH7, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.57 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human GAD67. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240876
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
glutamate decarboxylase 1 , CPSQ1 , GAD , SCP
Background
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX101881 WB Image
GAD67 antibody detects GAD67 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human GAD1-transfected 293T cells
7.5% SDS-PAGE
GAD67 antibody (GTX101881) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101881 IHC-P Image
GAD67 antibody detects GAD67 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101881 IHC-P Image
GAD67 antibody detects GAD67 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101881 ICC/IF Image
GAD67 antibody detects GAD67 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:500.Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX101881 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101881 ICC/IF Image
GAD67 antibody detects GAD67 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX101881 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101881 WB Image
Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101881 IHC-P Image
GAD67 antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101881 IHC-P Image
GAD67 antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101881 IHC-P Image
GAD67 antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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