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GAPDH antibody [6C5]

Anti-GAPDH antibody [6C5] used in Immunoprecipitation (IP). GTX28245
Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245
Anti-GAPDH antibody [6C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28245

Cat No. GTX28245

Host Mouse
Clonality Monoclonal
Clone Name 6C5
Isotype IgG1
Application WB, ICC/IF, IP
Reactivity Human, Mouse, Rat, Rabbit, Cat, Dog, Pig, Fish
Package
100 μg ($239)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 0.09 % sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4º C.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Immunogen

Rabbit muscle GAPDH

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Cytoplasm, cytosol,Nucleus,Cytoplasm, cytoskeleton

Database

Research Area

DATA IMAGES
Anti-GAPDH antibody [6C5] used in Immunoprecipitation (IP). GTX28245

GTX28245 IP Image

Immunoprecipitation of GAPDH from rat heart extract using anti-GAPDH MAb 6C5(GTX28245) (A).
Mixture of protein A-Sepharose with anti-GAPDH MAbs and tissue extract was incubated for 30 min at room temperature and precipitated by centrifugation. Pellet was washed with PBS, suspended in reducing electrophoresis sample buffer and heated for 5 minutes at 100 ºC. After centrifugation supernatant was loaded on gel and proteins were separated by SDS electrophoresis.
Lane 1: Human GAPDH (1 µg)
Lane 2: GAPDH immunoprecipitated from rat heart tissue extract
Lane 3: Only GTX28245 preincubated with Protein A Sepharose
Lane 4: Only Protein A Sepharose

Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245

GTX28245 WB Image

Heart extracts were prepared from different animal species. About 0.5 mg of homogenized wet cardiac tissue per track was loaded.
Lane 1: isolated human GAPDH, 0.5 µg
Lane 2: human heart tissue extract
Lane 3: pig heart tissue extract
Lane 4: goat heart tissue extract
Lane 5: bovine heart tissue extract
Lane 6: dog heart tissue extract
Lane 7: mouse heart tissue extract
Lane 8: rat heart tissue extract
Lane 9: rabbit heart tissue extract

Anti-GAPDH antibody [6C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28245

GTX28245 ICC/IF Image

Immunostaining of GAPDH in A-10 cell line (rat aortic smooth muscle cells). Cells were fixed by formalin and GAPDH was stained by:
GTX28245 (green colour)
F-actin microfilaments-bindingdye (red)
DNA-binding dye (dark blue)

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Application Reference
Package List Price ($)
$ 239