GeneTex
United States (US)

GAPDH antibody [6C5]

GAPDH-antibody-6C5-GTX28245-IP-1_18121410_164.jpg
GAPDH-antibody-6C5-GTX28245-WB-1_18121410_833.jpg
GAPDH-antibody-6C5-GTX28245-ICC_IF-1_18121410_374.jpg

Cat No. GTX28245

Host Mouse
Clonality Monoclonal
Clone Name 6C5
Isotype IgG1
Application WB, ICC/IF, IP, ELISA, Apuri, CC, Control, Puri
Reactivity Human, Mouse, Rat, Rabbit, Cat, Dog, Pig, Fish
APPLICATION

Application Note

AP: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 10 μg/ml. Gives a very strong band in Western Blotting in concentration 10 μg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 40.2 kDa). Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

36 kDa. ( Note )

Specificity/Sensitivity

This antibody can be used as a loading control antibody. GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. There is no cross-reaction with GAPDH from yeast.
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4, containing 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

12.8mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Immunogen

Rabbit muscle GAPDH.

Purification

Protein A purified
Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

G3PD G3PDH GAPD Glyceraldehyde 3 phosphate dehydrogenase Glyceraldehyde 3 phosphate dehydrogenase liver Glyceraldehyde 3 phosphate dehydrogenase muscle

Cellular Localization

Cytoplasmic

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age-related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed in almost all tissues at high levels, therefore becoming the marker of choice when a loading control in Western blotting is required. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Database

Research Area

DATA IMAGES
GAPDH-antibody-6C5-GTX28245-IP-1_18121410_164.jpg

GTX28245 IP Image

Immunoprecipitation of GAPDH from rat heart extract using anti-GAPDH MAb 6C5(GTX28245) (A).
Mixture of protein A-Sepharose with anti-GAPDH MAbs and tissue extract was incubated for 30 min at room temperature and precipitated by centrifugation. Pellet was washed with PBS, suspended in reducing electrophoresis sample buffer and heated for 5 minutes at 100 ºC. After centrifugation supernatant was loaded on gel and proteins were separated by SDS electrophoresis.
Lane 1: Human GAPDH (1 µg)
Lane 2: GAPDH immunoprecipitated from rat heart tissue extract
Lane 3: Only GTX28245 preincubated with Protein A Sepharose
Lane 4: Only Protein A Sepharose

GAPDH-antibody-6C5-GTX28245-WB-1_18121410_833.jpg

GTX28245 WB Image

Heart extracts were prepared from different animal species. About 0.5 mg of homogenized wet cardiac tissue per track was loaded.
Lane 1: isolated human GAPDH, 0.5 µg
Lane 2: human heart tissue extract
Lane 3: pig heart tissue extract
Lane 4: goat heart tissue extract
Lane 5: bovine heart tissue extract
Lane 6: dog heart tissue extract
Lane 7: mouse heart tissue extract
Lane 8: rat heart tissue extract
Lane 9: rabbit heart tissue extract

GAPDH-antibody-6C5-GTX28245-ICC_IF-1_18121410_374.jpg

GTX28245 ICC/IF Image

Immunostaining of GAPDH in A-10 cell line (rat aortic smooth muscle cells). Cells were fixed by formalin and GAPDH was stained by:
GTX28245 (green colour)
F-actin microfilaments-bindingdye (red)
DNA-binding dye (dark blue)

REFERENCE
Application Reference
Package List Price ($)
$ 239