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GAPDH antibody [6C5]

Orthogonal
Anti-GAPDH antibody [6C5] used in Immunoprecipitation (IP). GTX28245
Anti-GAPDH antibody [6C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28245
Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245
Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245
Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245
Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245
Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245
Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245
Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245
Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245
Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245
Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245
Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245
Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245
Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245
Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245
Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245
Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245

Cat. No. GTX28245

Reference 35

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6C5

Isotype

 IgG1

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat, Rabbit, Cat, Dog, Pig, Fish
Package
100 μg ($399)
See all GAPDH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Immunogen

Rabbit muscle GAPDH

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_370675

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Cytoplasm, cytosol,Nucleus,Cytoplasm, cytoskeleton

Database

Research Area

DATA IMAGES

Anti-GAPDH antibody [6C5] used in Immunoprecipitation (IP). GTX28245

GTX28245 IP Image

Immunoprecipitation of GAPDH from rat heart extract using anti-GAPDH MAb 6C5(GTX28245) (A).
Mixture of protein A-Sepharose with anti-GAPDH MAbs and tissue extract was incubated for 30 min at room temperature and precipitated by centrifugation. Pellet was washed with PBS, suspended in reducing electrophoresis sample buffer and heated for 5 minutes at 100 oC. After centrifugation supernatant was loaded on gel and proteins were separated by SDS electrophoresis.
Lane 1: Human GAPDH (1 μg)
Lane 2: GAPDH immunoprecipitated from rat heart tissue extract
Lane 3: Only GTX28245 preincubated with Protein A Sepharose
Lane 4: Only Protein A Sepharose

Anti-GAPDH antibody [6C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28245

GTX28245 ICC/IF Image

Immunostaining of GAPDH in A-10 cell line (rat aortic smooth muscle cells). Cells were fixed by formalin and GAPDH was stained by:
GTX28245 (green colour)
F-actin microfilaments-bindingdye (red)
DNA-binding dye (dark blue)

Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245

GTX28245 WB Image

Heart extracts were prepared from different animal species. About 0.5 mg of homogenized wet cardiac tissue per track was loaded.
Lane 1: isolated human GAPDH, 0.5 μg
Lane 2: human heart tissue extract
Lane 3: pig heart tissue extract
Lane 4: goat heart tissue extract
Lane 5: bovine heart tissue extract
Lane 6: dog heart tissue extract
Lane 7: mouse heart tissue extract
Lane 8: rat heart tissue extract
Lane 9: rabbit heart tissue extract
Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245

GTX28245 WB Image

Orthogonal

The data was published in the journal Nat Commun in 2020.PMID: 32647127

Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245

GTX28245 WB Image

The data was published in the journal Nat Commun in 2017. PMID: 28139683

Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245

GTX28245 WB Image

The data was published in the journal Nat Commun in 2017. PMID: 28139683

Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245

GTX28245 WB Image

The data was published in the journal Nat Commun in 2017. PMID: 28139683

Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245

GTX28245 WB Image

The data was published in the journal Nat Commun in 2017. PMID: 28139683

Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245

GTX28245 WB Image

The data was published in the journal Sci Rep in 2019. PMID: 31685876

Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245

GTX28245 WB Image

The data was published in the journal Sci Rep in 2019. PMID: 31685876

Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245

GTX28245 WB Image

The data was published in the journal Nat Commun in 2017. PMID: 28139683

Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245

GTX28245 WB Image

The data was published in the journal Nat Commun in 2017. PMID: 28139683

Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245

GTX28245 WB Image

The data was published in the journal Front Cell Neurosci in 2019.PMID: 31474830

Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245

GTX28245 WB Image

The data was published in the journal Nat Commun in 2020.PMID: 32647127

Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245

GTX28245 WB Image

The data was published in the journal Nat Commun in 2020.PMID: 32647127

Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245

GTX28245 WB Image

The data was published in the journal Nat Commun in 2020.PMID: 32647127

Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245

GTX28245 WB Image

The data was published in the journal Nat Commun in 2020.PMID: 32647127

Anti-GAPDH antibody [6C5] used in Western Blot (WB). GTX28245

GTX28245 WB Image

The data was published in the journal Nat Commun in 2020.PMID: 32647127

REFERENCE

Application Reference

Barbosa NS et al. J Virol 2023; 97 (1) : e0133122 Oropouche Virus Glycoprotein Topology and Cellular Requirements for Glycoprotein Secretion.
S Kurokawa et al. Int Heart J 2023; 64 : 453-461 Intravenous Semaphorin 3A Administration Maintains Cardiac Contractility and Improves Electrical Remodeling in a Mouse Model of Isoproterenol-Induced Heart Failure
Application : WB
Reactivity : Mouse
Matsuo R et al. JID Innov 2022; 2 (6) : 100141 Ninjurin1 Deletion in NG2-Positive Pericytes Prevents Microvessel Maturation and Delays Wound Healing.
Application : WB
Reactivity : Mouse
Lawson CD et al. Elife 2022; 11 Nuclear fascin regulates cancer cell survival.
Benedyk TH et al. J Biol Chem 2022; 298 (11) : 102589 Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the interorganelle transport protein CERT.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Barbosa NS et al. J Virol 2023; 97 (1):e0133122 Oropouche Virus Glycoprotein Topology and Cellular Requirements for Glycoprotein Secretion.
S Kurokawa et al. Int Heart J 2023; 64:453-461 Intravenous Semaphorin 3A Administration Maintains Cardiac Contractility and Improves Electrical Remodeling in a Mouse Model of Isoproterenol-Induced Heart Failure
Application : WB
Reactivity : Mouse
Radwitz J et al. Cell Mol Life Sci 2022; 79 (11):575 Tubb3 expression levels are sensitive to neuronal activity changes and determine microtubule growth and kinesin-mediated transport.
Application : WB
Reactivity : Human
Matsuo R et al. JID Innov 2022; 2 (6):100141 Ninjurin1 Deletion in NG2-Positive Pericytes Prevents Microvessel Maturation and Delays Wound Healing.
Application : WB
Reactivity : Mouse
Lawson CD et al. Elife 2022; 11 Nuclear fascin regulates cancer cell survival.
Benedyk TH et al. J Biol Chem 2022; 298 (11):102589 Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the interorganelle transport protein CERT.
Application : WB
Reactivity : Human
Gonzalo P Solis et al. Nat Commun 2022; 13 (1):2072 Local and substrate-specific S-palmitoylation determines subcellular localization of Gαo.
Application : WB
Mert Duman et al. Biomedicines 2022; 10 (6) Theophylline Induces Remyelination and Functional Recovery in a Mouse Model of Peripheral Neuropathy.
Application : WB
Reactivity : Mouse
Tomasz H Benedyk et al. PLoS Pathog 2021; 17 (8):e1009824 pUL21 is a viral phosphatase adaptor that promotes herpes simplex virus replication and spread.
Application : WB
Morita A et al. Life (Basel) 2021; 11 (10) A Novel RNA Synthesis Inhibitor, STK160830, Has Negligible DNA-Intercalating Activity for Triggering A p53 Response, and Can Inhibit p53-Dependent Apoptosis.
Application : WB
Reactivity : Human
Koinuma S et al. Neurobiol Aging 2021; 106:268-281 Aging induces abnormal accumulation of Aβ in extracellular vesicle and/or intraluminal membrane vesicle-rich fractions in nonhuman primate brain.
Application : WB
Reactivity : Rat
Duman M et al. Nat Commun 2020; 11 (1):3420 EEF1A1 deacetylation enables transcriptional activation of remyelination.
Beauchemin M et al. Pharmacol Res 2020; 152:104589 Exploring mechanisms of increased cardiovascular disease risk with antipsychotic medications: Risperidone alters the cardiac proteomic signature in mice.
Application : WB
Reactivity : Mouse
Shadle SC et al. Hum Mol Genet 2019; 28 (23):3997-4011 DUX4-induced bidirectional HSATII satellite repeat transcripts form intranuclear double-stranded RNA foci in human cell models of FSHD.
Roesler MK et al. Front Cell Neurosci 2019; 13:330 Myosin XVI Regulates Actin Cytoskeleton Dynamics in Dendritic Spines of Purkinje Cells and Affects Presynaptic Organization.
Application : WB
Tang X et al. Sci Rep 2019; 9 (1):15108 Tumour-Secreted Hsp90帢 on External Surface of Exosomes Mediates Tumour - Stromal Cell Communication via Autocrine and Paracrine Mechanisms.
Application : WB
Reactivity : Human
Lombino FL et al. Sci Rep 2019; 9 (1):15940 The Microtubule Severing Protein Katanin Regulates Proliferation of Neuronal Progenitors in Embryonic and Adult Neurogenesis.
Application : WB
Reactivity : Mouse
Sean C. Shadle et al. bioRxiv 2019; (Epub) DUX4-induced bidirectional HSATII satellite repeat transcripts form intranuclear double stranded RNA foci in human cell models of FSHD.
Application : WB
Reactivity : Human
Chew GL et al. Dev Cell 2019; DUX4 Suppresses MHC Class I to Promote Cancer Immune Evasion and Resistance to Checkpoint Blockade.
Application : WB
Reactivity : Human
de Wispelaere M et al. Nat Commun 2019; 10 (1):3468 Small molecule degraders of the hepatitis C virus protease reduce susceptibility to resistance mutations.
Vaqui? A et al. Cell Rep 2019; 27 (11):3152-3166.e7 Injured Axons Instruct Schwann Cells to Build Constricting Actin Spheres to Accelerate Axonal Disintegration.
Application : WB
Reactivity : Mouse
Patil AV et al. J Biol Chem 2017; 292 (10):4313-4325 Ribosomal Protein S3 Negatively Regulates Unwinding Activity of RecQ-like Helicase 4 through Their Physical Interaction.
Guo J et al. Mol Cell Biol 2017; 37 (19) PRAS40 Connects Microenvironmental Stress Signaling to Exosome-Mediated Secretion.
Brugger V et al. Nat Commun 2017; 8:14272 Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration.
Dong H et al. Sci Rep 2016; 6:20605 Breast Cancer MDA-MB-231 Cells Use Secreted Heat Shock Protein-90alpha (Hsp90α) to Survive a Hostile Hypoxic Environment.
Application : WB
Reactivity : Human
Chen CW et al. Cell Rep 2016; 16 (5):1287-99 The Impact of dUTPase on Ribonucleotide Reductase-Induced Genome Instability in Cancer Cells.
Application : ICC/IF
Reactivity : Human
Meng J et al. J Virol 2015; 90 (1):245-53 Respiratory Syncytial Virus Attachment Glycoprotein Contribution to Infection Depends on the Specific Fusion Protein.
Application : WB
Reactivity : Human
Jayaprakash P et al. J Cell Sci 2015; 128 (8):1475-80 Hsp90α and Hsp90β together operate a hypoxia and nutrient paucity stress-response mechanism during wound healing.
Rubin B et al. Cancer Invest 2015; (Epub) Mitogen-Activated Protein Kinase Pathway: Genetic Analysis of 95 Adrenocortical Tumors.
Application : WB
Reactivity : Human
Meng J et al. MBio 2014; 5 (5):e01704-14 Refining the balance of attenuation and immunogenicity of respiratory syncytial virus by targeted codon deoptimization of virulence genes.
Application : WB
Reactivity : Human
Fuerst E et al. Allergy 2014; Sphingosine-1-phosphate induces pro-remodelling response in airway smooth muscle cells.
Application : WB
Reactivity : Human
Kiryu M et al. Europace 2012; 14 (8):1206-14 Angiotensin II-mediated up-regulation of connective tissue growth factor promotes atrial tissue fibrosis in the canine atrial fibrillation model.
Application : WB
Reactivity : Dog
Hsu SP et al. Atherosclerosis 2011; 217 (1):30560 Extra-nuclear activation of progesterone receptor in regulating arterial smooth muscle cell migration.
Application : WB
Reactivity : Rat
Clone Reference
Commander R et al. Nat Commun 2020; 11 (1):1533 Subpopulation targeting of pyruvate dehydrogenase and GLUT1 decouples metabolic heterogeneity during collective cancer cell invasion.
Bajpai R et al. Oncogene 2016; 35 (30):3955-64 Targeting glutamine metabolism in multiple myeloma enhances BIM binding to BCL-2 eliciting synthetic lethality to venetoclax.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399