AP: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 10 μg/ml. Gives a very strong band in Western Blotting in concentration 10 μg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 40.2 kDa). Optimal dilutions/concentrations should be determined by the end user.
This antibody can be used as a loading control antibody. GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. There is no cross-reaction with GAPDH from yeast.
Phosphate-buffered saline, pH 7.4, containing 0.1% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
12.8mg/ml(Please refer to the vial label for the specific concentration.)
Rabbit muscle GAPDH.
Protein A purified
Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
G3PD G3PDH GAPD Glyceraldehyde 3 phosphate dehydrogenase Glyceraldehyde 3 phosphate dehydrogenase liver Glyceraldehyde 3 phosphate dehydrogenase muscle
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age-related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed in almost all tissues at high levels, therefore becoming the marker of choice when a loading control in Western blotting is required. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.